Androgen Receptor-dependent Expression of Low-density Lipoprotein Receptor-related Protein 6 is Necessary for Prostate Cancer Cell Proliferation.
10.4196/kjpp.2015.19.3.235
- Author:
Eun PARK
1
;
Eun Kyoung KIM
;
Minkyoung KIM
;
Jung Min HA
;
Young Whan KIM
;
Seo Yeon JIN
;
Hwa Kyoung SHIN
;
Hong Koo HA
;
Jeong Zoo LEE
;
Sun Sik BAE
Author Information
1. Department of Urology, Wallace Memorial Baptist Hospital, Busan 609-728, Korea.
- Publication Type:Original Article
- Keywords:
Androgen receptor;
LRP6;
Prostate cancer;
Testosterone;
Wnt
- MeSH:
Cell Proliferation*;
Low Density Lipoprotein Receptor-Related Protein-6*;
Prostatic Neoplasms*;
Receptors, Androgen;
Testosterone
- From:The Korean Journal of Physiology and Pharmacology
2015;19(3):235-240
- CountryRepublic of Korea
- Language:English
-
Abstract:
Androgen receptor (AR) signaling is important for prostate cancer (PCa) cell proliferation. Here, we showed that proliferation of hormone-sensitive prostate cancer cells such as LNCaP was significantly enhanced by testosterone stimulation whereas hormone-insensitive prostate cancer cells such as PC3 and VCaP did not respond to testosterone stimulation. Blocking of AR using bicalutamide abolished testosterone-induced proliferation of LNCaP cells. In addition, knockdown of AR blocked testosterone-induced proliferation of LNCaP cells. Basal expression of low-density lipoprotein receptor-related protein 6 (LRP6) was elevated in VCaP cells whereas stimulation of testosterone did not affect the expression of LRP6. However, expression of LRP6 in LNCaP cells was increased by testosterone stimulation. In addition, knockdown of LRP6 abrogated testosterone-induced proliferation of LNCaP cells. Given these results, we suggest that androgen-dependent expression of LRP6 plays a crucial role in hormone-sensitive prostate cancer cell proliferation.
