Effects of Chlorhexidine digluconate on Rotational Rate of n- (9-Anthroyloxy)stearic acid in Model Membranes of Total Lipids Extracted from Porphyromonas gingivalis Outer Membranes.
- Author:
Hye Ock JANG
1
;
Dong Won KIM
;
Byeong Ill KIM
;
Hong Gu SIM
;
Young Ho LEE
;
Jong Hwa LEE
;
Jung Ha BAE
;
Moon Kyoung BAE
;
Tae Hyuk KWON
;
Il YUN
Author Information
1. Department of Dental Pharmacology and Biophysics, College of Dentistry and Research Institute for Oral Biotechnology, Pusan National University, Busan 602-739, Korea.
- Publication Type:Original Article
- Keywords:
Liposomes;
Chlorhexidine digluconate;
Fluorescence probe technique;
Membrane hydrocarbon interior;
Membrane interface;
Rotational rate;
n- (9-anthroyloxy)stearic acid
- MeSH:
Chlorhexidine*;
Fluorescence Polarization;
Liposomes;
Membrane Lipids;
Membranes*;
Phospholipids;
Porphyromonas gingivalis*;
Porphyromonas*;
Thiram;
Unilamellar Liposomes
- From:The Korean Journal of Physiology and Pharmacology
2004;8(2):83-88
- CountryRepublic of Korea
- Language:English
-
Abstract:
The purpose of this study was to provide a basis for studying the molecular mechanism of pharmacological action of chlorhexidine digluconate. Large unilamellar vesicles (OPGTL) were prepared with total lipids extracted from cultured Porphyromonas gingivalis outer membranes (OPG). The anthroyloxy probes were located at a graded series of depths inside a membrane, depending on its substitution position (n) in the aliphatic chain. Fluorescence polarization of n- (9-anthroyloxy)stearic acid was used to examine effects of chlorhexidine digluconate on differential rotational mobility, while changing the probes' substitution position (n) in the membrane phospholipids aliphatic chain. Magnitude of the rotational mobility of the intact six membrane components differed depending on the substitution position in the descending order of 16- (9-anthroyloxy)palmitic acid (16-AP), 12, 9, 6, 3 and 2- (9-anthroyloxy)stearic acid (12-AS, 9-AS, 6-AS, 3-AS and 2-AS). Chlorhexidine digluconate increased in a dose-dependent manner the rate of rotational mobility of hydrocarbon interior of the OPGTL prepared with total lipids extracted from cultured OPG, but decreased the mobility of membrane interface of the OPGTL. Disordering or ordering effects of chlorhexidine digluconate on membrane lipids may be responsible for some, but not all of its bacteriostatic and bactericidal actions.
