Multiple Regulation of Roundabout (Robo) Phosphorylation in a Heterologous Cell System.
- Author:
Hwan Tae PARK
1
Author Information
1. Department of Physiology and Institute of Medical Science, College of Medicine, Dong-A University, Busan 602-714, Korea.
- Publication Type:Original Article
- Keywords:
Robo;
Slit;
Axon guidance;
Protein kinases;
Tyrosine phosphorylation
- MeSH:
Animals;
Brain;
Calcium;
Colforsin;
Cyclic AMP-Dependent Protein Kinases;
Humans;
Kidney;
Neurons;
Phosphorylation*;
Phosphotransferases;
Protein Kinase C;
Protein Kinases;
Rats;
Receptor, EphA1;
Receptors, Eph Family;
Transfection;
Tyrosine
- From:The Korean Journal of Physiology and Pharmacology
2004;8(2):111-115
- CountryRepublic of Korea
- Language:English
-
Abstract:
Roundabout (Robo) is the transmembrane receptor for slit, the neuronal guidance molecule. In this study, the tyrosine phosphorylation of Robo was observed in Robo-transfected human embryonic kidney cells and developing rat brains, and found to be increased by the treatment with protein kinase A activator, forskolin. In contrast, protein kinase C activation by phorbol-12-myristate-13-acetate decreased the phosphorylation of Robo. Intracellular calcium was required for the tyrosine phosphorylation. Furthermore, the transfection of an Eph receptor tyrosine kinase dramatically enhanced the tyrosine phosphorylation. These findings indicate that the tyrosine phosphorylation of Robo is regulated by multiple mechanisms, and that Eph receptor kinases may play a role in the regulation of tyrosine phosphorylation of Robo in the rat brain.
