Alternative Isoforms of TonEBP with Variable N-termini are Expressed in Mammalian Cells.
- Author:
Hyo Shin KIM
1
;
Sook Jin SON
;
Seon Nyo KIM
;
Yong Duk KIM
;
Kwang Jin KIM
;
Byeong Hwa JEON
;
Jin Bong PARK
;
Sang Do LEE
Author Information
1. Department of Physiology, College of Medicine, Research Institute for Medical Sciences, Chungnam National University, Daejeon 301-131, Korea. sdlee@ cnu.ac.kr
- Publication Type:Original Article
- Keywords:
TonEBP;
Hypertonicity;
Alternative splicing
- MeSH:
Aldehyde Reductase;
Alternative Splicing;
Antibodies;
Membrane Transport Proteins;
Osmolar Concentration;
Protein Isoforms*;
RNA, Messenger
- From:The Korean Journal of Physiology and Pharmacology
2007;11(3):135-138
- CountryRepublic of Korea
- Language:English
-
Abstract:
Hypertonicity imposes a great deal of stress to cells since it causes rise in cellular ionic strength, which can be reduced by the accumulation of compatible osmolytes. TonEBP plays a central role in the cellular accumulation of compatible osmolytes via transcriptional stimulation of membrane transporters and aldose reductase. Alternatively spliced forms of TonEBP mRNA have previously been reported and two of them showed different transcriptional activity. In the present study, isoform-specific antibodies were produced to confirm the translation of the spliced mRNA to protein. TonEBP was immunoprecipitated by using anti-TonEBP antibody and then immunoblotted using anti-TonEBP or isoform specific antibodies to find out the expression profile of TonEBP isoforms in basal or stimulated condition. From these results, we conclude that all TonEBP isoforms are expressed in mammalian cells and their expression patterns are not same in every cells.
