Identification of Differentially Expressed Genes in Murine Hippocampus by Modulation of Nitric Oxide in Kainic Acid-induced Neurotoxic Animal Model.
- Author:
Yo Ahn SUH
1
;
O Min KWON
;
So Young YIM
;
Hee Jae LEE
;
Sung Soo KIM
Author Information
1. Department of Pharmacology, College of Medicine, Kangwon National University, Chuncheon 200-701, Korea. ksslsy@kangwon.ac.kr
- Publication Type:Original Article
- Keywords:
Excitotoxicity;
Kainic acid;
L-NAME;
Differential display PCR;
Hippocampus
- MeSH:
Animals*;
Cell Death;
Clathrin;
Consensus;
Hippocampus*;
Kainic Acid;
Mice;
Models, Animal*;
Neurons;
NG-Nitroarginine Methyl Ester;
Nitric Oxide Synthase;
Nitric Oxide*;
Polymerase Chain Reaction;
Proton-Translocating ATPases
- From:The Korean Journal of Physiology and Pharmacology
2007;11(4):149-154
- CountryRepublic of Korea
- Language:English
-
Abstract:
Kainic acid (KA) causes neurodegeneration, but no consensus has been reached concerning its mechanism. Nitric oxide may be a regulator of the mechanism. We identified differentially expressed genes in the hippocampus of mice treated with kainic acid, together with or without L-NAME, a nonselective nitric oxide synthase inhibitor, using a new differential display PCR method based on annealing control primers. Eight genes were identified, including clathrin light polypeptide, TATA element modulatory factor 1, neurexin III, ND4, ATPase, H+ transporting, V1 subunit E isoform 1, and N-myc downstream regulated gene 2. Although the functions of these genes and their products remain to be determined, their identification provides insight into the molecular mechanism(s) involved in KA-induced neuronal cell death in the hippocampal CA3 area.
