Scopoletin from Cirsium setidens Increases Melanin Synthesis via CREB Phosphorylation in B16F10 Cells.
10.4196/kjpp.2014.18.4.307
- Author:
Mi Ja AHN
1
;
Sun Jung HUR
;
Eun Hyun KIM
;
Seung Hoon LEE
;
Jun Seob SHIN
;
Myo Kyoung KIM
;
James A UCHIZONO
;
Wan Kyunn WHANG
;
Dong Seok KIM
Author Information
1. Department of Pharmacognosy, Chung-Ang University College of Pharmacy, Seoul 156-756, Korea.
- Publication Type:Original Article
- Keywords:
Cirsium setidens;
CREB;
MITF;
Scopoletin;
Tyrosinase
- MeSH:
Blotting, Western;
Cell-Free System;
Cirsium*;
Cyclic AMP Response Element-Binding Protein;
Melanins*;
Microphthalmia-Associated Transcription Factor;
Monophenol Monooxygenase;
Phosphorylation*;
Scopoletin*;
Vitiligo
- From:The Korean Journal of Physiology and Pharmacology
2014;18(4):307-311
- CountryRepublic of Korea
- Language:English
-
Abstract:
In this study, we isolated scopoletin from Cirsium setidens Nakai (Compositae) and tested its effects on melanogenesis. Scopoletin was not toxic to cells at concentrations less than 50 microM and increased melanin synthesis in a dose-dependent manner. As melanin synthesis increased, scopoletin stimulated the total tyrosinase activity, the rate-limiting enzyme of melanogenesis. In a cell-free system, however, scopoletin did not increase tyrosinase activity, indicating that scopoletin is not a direct activator of tyrosinase. Furthermore, Western blot analysis showed that scopoletin stimulated the production of microphthalmia-associated transcription factor (MITF) and tyrosinase expression via cAMP response element-binding protein (CREB) phosphorylation in a dose-dependent manner. Based on these results, preclinical and clinical studies are needed to assess the use of scopoletin for the treatment of vitiligo.
