Fucoidan Promotes the Reconstruction of Skin Equivalents.
10.4196/kjpp.2014.18.4.327
- Author:
Yu Seok SONG
1
;
Hailan LI
;
Marie Carmel BALCOS
;
Hye Young YUN
;
Kwang Jin BAEK
;
Nyoun Soo KWON
;
Hye Ryung CHOI
;
Kyoung Chan PARK
;
Dong Seok KIM
Author Information
1. Department of Biochemistry, Chung-Ang University College of Medicine, Seoul 156-756, Korea. ds_kim@cau.ac.kr
- Publication Type:Original Article
- Keywords:
Fibroblasts;
Fucoidan;
Proliferation;
Skin equivalent
- MeSH:
Blotting, Western;
Collagen Type I;
Cyclin D1;
Elastin;
Fibroblasts;
Fibronectins;
Humans;
Proliferating Cell Nuclear Antigen;
Skin*
- From:The Korean Journal of Physiology and Pharmacology
2014;18(4):327-331
- CountryRepublic of Korea
- Language:English
-
Abstract:
In this study we investigated the effects of fucoidan on the proliferation of fibroblasts and the reconstruction of a skin equivalent (SE). Fucoidan significantly stimulated the proliferation of CCD-25Sk human fibroblasts and Western blot analysis demonstrated that fucoidan markedly increased the expression of cyclin D1 and decreased the expression of p27. Fucoidan was used to reconstruct SE. Immunohistochemical staining showed that the addition of fucoidan to dermal equivalents increased expression of proliferating cell nuclear antigen (PCNA) and p63. In addition, expression of alpha6-integrin was significantly increased by fucoidan, whereas expression of beta1-integrin, type 1 collagen, elastin, fibronectin did not markedly change. These results suggest that fucoidan has positive effects on epidermal reconstruction and will therefore be beneficial in the reconstruction of SE.
