Ellagic acid plays a protective role against UV-B-induced oxidative stress by up-regulating antioxidant components in human dermal fibroblasts.
10.4196/kjpp.2016.20.3.269
- Author:
Beomyeol BAEK
1
;
Su Hee LEE
;
Kyunghoon KIM
;
Hye Won LIM
;
Chang Jin LIM
Author Information
1. Department of Biochemistry, College of Natural Sciences, Kangwon National University, Chuncheon 24341, Korea. cjlim@kangwon.ac.kr
- Publication Type:Original Article
- Keywords:
Ellagic acid;
Glutathione;
Superoxide dismutase;
UV-B radiation
- MeSH:
Cell Survival;
Ellagic Acid*;
Fibroblasts*;
Glutathione;
Humans*;
Matrix Metalloproteinase 2;
Oxidative Stress*;
Plants;
Reactive Oxygen Species;
Skin;
Superoxide Dismutase
- From:The Korean Journal of Physiology and Pharmacology
2016;20(3):269-277
- CountryRepublic of Korea
- Language:English
-
Abstract:
Ellagic acid (EA), an antioxidant polyphenolic constituent of plant origin, has been reported to possess diverse pharmacological properties, including anti-inflammatory, anti-tumor and immunomodulatory activities. This work aimed to clarify the skin anti-photoaging properties of EA in human dermal fibroblasts. The skin anti-photoaging activity was evaluated by analyzing the reactive oxygen species (ROS), matrix metalloproteinase-2 (MMP-2), total glutathione (GSH) and superoxide dismutase (SOD) activity levels as well as cell viability in dermal fibroblasts under UV-B irradiation. When fibroblasts were exposed to EA prior to UV-B irradiation, EA suppressed UV-B-induced ROS and proMMP-2 elevation. However, EA restored total GSH and SOD activity levels diminished in fibroblasts under UV-B irradiation. EA had an up-regulating activity on the UV-B-reduced Nrf2 levels in fibroblasts. EA, at the concentrations used, was unable to interfere with cell viabilities in both non-irradiated and irradiated fibroblasts. In human dermal fibroblasts, EA plays a defensive role against UV-B-induced oxidative stress possibly through an Nrf2-dependent pathway, indicating that this compound has potential skin antiphotoaging properties.
