The effect of progesterone and 17-β estradiol on membrane-bound HLA-G in adipose derived stem cells.
10.4196/kjpp.2016.20.4.341
- Author:
Akram MOSLEHI
1
;
Batool HASHEMI-BENI
;
Azam MOSLEHI
;
Maryam Ali AKBARI
;
Minoo ADIB
Author Information
1. Department of Immunology, Medical School, Isfahan University of Medical Sciences, Isfahan 81746-73461, Iran. adib@med.mui.ac.ir
- Publication Type:Original Article
- Keywords:
Adipose derived stem cell;
Estradiol;
Membrane-bound HLA-G;
Progesterone
- MeSH:
Cell Culture Techniques;
Cell Line;
Estradiol*;
Flow Cytometry;
HLA-G Antigens*;
Phenolsulfonphthalein;
Pregnancy;
Progesterone*;
Stem Cells*
- From:The Korean Journal of Physiology and Pharmacology
2016;20(4):341-346
- CountryRepublic of Korea
- Language:English
-
Abstract:
Membrane-bound HLA-G (mHLA-G) discovery on adipose derived stem cells (ADSCs) as a tolerogenic and immunosuppressive molecule was very important. Many documents have shown that HLA-G expression can be controlled via some hormones such as progesterone (P4) and estradiol (E2). Therefore, this study was designed to evaluate progesterone and estradiol effects on mHLA-G in ADSCs at restricted and combination concentrations. Three independent cell lines were cultured in complete free phenol red DMEM and subcultured to achieve suffi cient cells. These cells were treated with P4, E2 and P4 plus E2 at physiologic and pregnancy concentrations for 3 days in cell culture conditions. The HLA-G positive ADSCs was measured via monoclonal anti HLA-G-FITC/MEMG-09 by means of flow cytometry in nine groups. Data were analyzed by one way ANOVA and Tukey's post hoc tests. There were no signifi cant values of the mean percentage of HLA-G positive cells in E2-treated and the combination of P4 plus E2-treated ADSCs compared to control cells (p value>0.05) but P4 had a signifi cant increase on mHLA-G in ADSCs (p value<0.05). High P4 concentration increased mHLA-G but E2 and the combination of P4 plus E2 could not change mHLA-G on ADSCs.
