Effects of ethanol on Na+-dependent solute uptake in rabbit renal brush-border membrane vesicles.
- Author:
Yong Keun KIM
1
;
Sun Hee KO
;
Jae Suk WOO
;
Jin Sup JUNG
;
Sang Ho LEE
Author Information
1. Department of Physiology, College of Medicine, Pusan National University, Pusan, 602-739 South Korea.
- Publication Type:Original Article
- Keywords:
Ethanol;
Phosphate uptake;
Glucose uptake;
Brush-border membrane vesicles;
Rabbit kidney
- MeSH:
Carrier Proteins;
Ethanol*;
Glucose;
Ion Transport;
Membranes*;
Phlorhizin;
Succinic Acid
- From:The Korean Journal of Physiology and Pharmacology
1999;3(2):191-198
- CountryRepublic of Korea
- Language:English
-
Abstract:
This study was undertaken to examine the effect of ethanol on Na+-dependent transport systems (glucose, phosphate, and dicarboxylate) in renal brush-border membrane vesicles (BBMV). Ethanol inhibited Na+-dependent uptakes of glucose, phosphate, and succinate in a dose-dependent manner, but not the uptakes of Na+-independent. The H+/TEA antiport was reduced by 8% ethanol. Kinetic analysis showed that ethanol caused a decrease in Vmax of three transport systems, leaving Km values unchanged. Ethanol decreased phlorizin binding, which was closely correlated with the decrease in Vmax of Na+-glucose uptake. These results indicate that ethanol inhibits Na+-dependent uptakes of glucose, phosphate, and dicaboxylate and that the reduction in Vmax of Na+-glucose uptake is caused by a decrease in the number of active carrier proteins in the membrane.
