The Influence of Alpha-fetoprotein on Natural Suppressor Cell Activity and Ehrlich Carcinoma Growth.
10.4196/kjpp.2008.12.4.193
- Author:
Nikolai Nikolaevich BELYAEV
1
;
Andrei Yurievich BOGDANOV
;
Philipp Georgievich SAVVULIDI
;
Vladimir Konstantinovich KRASNOSHTANOV
;
Raikhan Tleulievna TLEULIEVA
;
Gabit Kaimovich ALIPOV
;
Ichiro SEKINE
;
Jun Sang BAE
;
Jeong Beom LEE
;
Young Ki MIN
;
Hun Mo YANG
Author Information
1. Institute of Molecular Biology and Biochemistry, Kazakhstan Medical University, Almaty 050022, Kazakhstan.
- Publication Type:Original Article
- Keywords:
Alpha-fetoprotein (AFP);
Natural suppressor (NS) cells;
Natural killer cells;
Ehrlich carcinoma (EC)
- MeSH:
alpha-Fetoproteins;
Animals;
Bone Marrow;
Centrifugation, Isopycnic;
Interleukin-3;
Killer Cells, Natural;
Mice;
Mice, Inbred CBA;
Povidone;
Silicon Dioxide
- From:The Korean Journal of Physiology and Pharmacology
2008;12(4):193-197
- CountryRepublic of Korea
- Language:English
-
Abstract:
The influence of alpha-fetoprotein (AFP) on the bone marrow (BM) natural suppressor (NS) cells of intact Ehrlich carcinoma -bearing CBA mice was studied. Bone marrow NS cells were fractionated into three fractions by isopycnic centrifugation on percoll gradients: NS1 (rho=1.080 g/ml), NS2 (rho=1.090 g/ml) and NS3 (1.100>rho>1.090 g/ml). These fractions were highly different in their sensitivity to known NS cell inductors (interleukin (IL)-2, IL-3 or histamine). None of the NS fractions isolated from the intact mice spontaneously produced antiproliferative activity, however, they showed a high level of NS (antiproliferative and natural killer cell inhibitory) activity under the influence of AFP. A single injection of AFP to intact mice led to an increase of spontaneous NS activity and the inhibition of natural killer cell activity. NS activity, especially NS2, was increased in when tumor cells were subcutaneously inoculated three days after AFP injection. In the AFP-treated mice, the tumor mass at 14 days was 60% larger than that in the untreated mice. Our data confirmed that AFP is a tumor marker that can inhibit cancer immunity and plays a role in cancer pathogenesis.
