Antinociception Effect and Mechanisms of Campanula Punctata Extract in the Mouse.
10.4196/kjpp.2010.14.5.285
- Author:
Soo Hyun PARK
1
;
Yun Beom SIM
;
Soon Sung LIM
;
Jin Kyu KIM
;
Jin Koo LEE
;
Hong Won SUH
Author Information
1. Department of Pharmacology, College of Medicine, Hallym University, Chuncheon 200-702, Korea. hwsuh@hallym.ac.kr
- Publication Type:Original Article
- Keywords:
Campanula punctata;
Anti-nociception;
Inflammatory pain;
alpha2 adrenoceptor
- MeSH:
Animals;
Campanulaceae;
Methysergide;
Mice;
Mice, Inbred ICR;
Naloxone;
Reaction Time;
Saponins;
Substance P;
Yohimbine
- From:The Korean Journal of Physiology and Pharmacology
2010;14(5):285-289
- CountryRepublic of Korea
- Language:English
-
Abstract:
In the present study, the antinociceptive profiles of Campanula punctata extract were examined in ICR mice. The Campanula punctata contain a large dose of saponin. Campanula punctata extract administered orally (200 mg/kg) showed an antinociceptive effect as measured by the tail-flick and hot-plate tests. In addition, Campanula punctata extract attenuated the writhing numbers in the acetic acid-induced writhing test. Furthermore, the cumulative nociceptive response time for intrathecal (i.t.) injection of substance P (0.7 microgram) was diminished by Campanula punctata extract. Intraperitoneal (i.p.) pretreatment with yohimbine (alpha2-adrenergic receptor antagonist) attenuated antinociceptive effect induced by Campanula punctata extract in the writhing test. However, naloxone (opioid receptor antagonist) or methysergide (5-HT serotonergic receptor antagonist) did not affect antinociception induced by Campanula punctata extract in the writhing test. Our results suggest that Campanula punctata extract shows an antinociceptive property in various pain models. Furthermore, this antinociceptive effect of Campanula punctata extract may be mediated by alpha2-adrenergic receptor, but not opioidergic and serotonergic receptors.
