Effects of Erythropoietin on Memory Deficits and Brain Oxidative Stress in the Mouse Models of Dementia.
10.4196/kjpp.2010.14.5.345
- Author:
Rohit KUMAR
1
;
Amteshwar Singh JAGGI
;
Nirmal SINGH
Author Information
1. Department of Pharmaceutical Sciences and Drug Research, Punjabi University, Patiala (Punjab), PIN-147002, India. nirmal_puru@rediffmail.com
- Publication Type:Original Article
- Keywords:
Erythropoietin;
Memory;
Scopolamine;
Streptozotocin;
Morris water-maze
- MeSH:
Acetylcholinesterase;
Animals;
Brain;
Dementia;
Erythropoietin;
Glutathione;
Learning;
Memory;
Memory Disorders;
Mice;
Oxidative Stress;
Scopolamine Hydrobromide;
Streptozocin;
Thiobarbituric Acid Reactive Substances
- From:The Korean Journal of Physiology and Pharmacology
2010;14(5):345-352
- CountryRepublic of Korea
- Language:English
-
Abstract:
The present study was undertaken to explore the potential of erythropoietin in memory deficits of mice. Memory impairment was produced by scopolamine (0.5 mg/kg, i.p.) and intracerebroventricular streptozotocin (i.c.v STZ, 3 mg/kg, 10 microliter, 1st and 3rd day) in separate groups of animals. Morris water-maze test was employed to assess learning and memory. The levels of brain thio-barbituric acid reactive species (TBARS) and reduced glutathione (GSH) were estimated to assess degree of oxidative stress. Brain acetylcholinesterase enzyme (AChE) activity was also measured. Scopolamine/streptozotocin administration induced significant impairment of learning and memory in mice as indicated by marked decrease in Morris water-maze performance. Scopolamine/streptozotocin administration also produced a significant enhancement of brain AChE activity and brain oxidative stress (an increase in TBARS and a decrease in GSH) levels. Treatment of erythropoietin (500 and 1,000 IU/Kg i.p.) significantly reversed scopolamine- as well as streptozotocin-induced learning and memory deficits along with attenuation of those-induced rise in brain AChE activity and brain oxidative stress levels. It may be concluded that erythropoietin exerts a beneficial effect in memory deficits of mice possibly through its multiple actions including potential anti-oxidative effect.
