Transition metal induces apoptosis in MC3T3E1 osteoblast: Evidence of free radical release.
- Author:
Han Jung CHAE
1
;
Soo Wan CHAE
;
Jang Sook KANG
;
Dong Hyeon YUN
;
Byung Gwan BANG
;
Mi Ra KANG
;
Hyung Min KIM
;
Hyung Ryong KIM
Author Information
1. Department of Dental Pharmacology, School of Dentistry, Wonkwang University, Iksan, Chonbuk, South Korea.
- Publication Type:Original Article
- MeSH:
Acetylcysteine;
Apoptosis*;
Cadmium;
Catalase;
Chromatin;
Cysteine;
Cysteine Proteases;
Dimethyl Sulfoxide;
DNA Fragmentation;
Free Radicals;
Glutathione;
Ions;
Manganese;
Metabolism;
Metals;
Nitroblue Tetrazolium;
Osteoblasts*;
Reactive Oxygen Species;
Selenium;
Superoxide Dismutase
- From:The Korean Journal of Physiology and Pharmacology
2000;4(1):47-54
- CountryRepublic of Korea
- Language:English
-
Abstract:
Transition metal ions including Se2+, Cd2+, Hg2+ or Mn2+ have been thought to disturb the bone metabolism directly. However, the mechanism for the bone lesion is unknown. In this study, we demonstrated that MC3T3E1 osteoblasts, exposed to various transition metal ions; selenium, cadmium, mercury or manganese, generated massive amounts of reactive oxygen species (ROS). The released ROS were completely quenched by free radical scavengers-N-acetyl cysteine (NAC), reduced glutathione (GSH), or superoxide dismutase (SOD). First, we have observed that selenium (10 micrometer), cadmium (100 micrometer), mercury (100 micrometer) or manganese (1 mM) treatment induced apoptotic phenomena like DNA fragmentation, chromatin condensation and caspase-3-like cysteine protease activation in MC3T3E1 osteoblasts. Concomitant treatment of antioxidant; N-acetyl-L-cysteine (NAC), reduced-form glutathione (GSH), or superoxide dismutase (SOD), prevented apoptosis induced by each of the transition metal ions. Catalase or dimethylsulfoxide (DMSO) has less potent inhibitory effect on the apoptosis, compared with NAC, GSH or SOD. In line with the results, nitroblue tetrazolium (NBT) stain shows that each of the transition metals is a potent source of free radicals in MC3T3E1 osteoblast. Our data show that oxidative damage is associated with the induction of apoptosis in MC3T3E1 osteoblasts following Se2+, Cd2+, Hg2+ or Mn2+ treatment.
