Effect of sedative dose of propofol on neuronal damage after transient forebrain ischemia in Mongolian gerbils.
- Author:
Seong Ryong LEE
1
Author Information
1. Department of Pharmacology, Keimyung University School of Medicine, 194 Dongsan-dong, Taegu, South Korea.
- Publication Type:Original Article
- MeSH:
Animals;
Brain;
Carotid Arteries;
Cell Death;
DNA Nucleotidylexotransferase;
Gerbillinae*;
In Situ Nick-End Labeling;
Ischemia*;
Neurons*;
Propofol*;
Prosencephalon*;
Pyramidal Cells
- From:The Korean Journal of Physiology and Pharmacology
2000;4(1):73-79
- CountryRepublic of Korea
- Language:English
-
Abstract:
This study investigated whether propofol, an intravenous, non-barbiturate anesthetic, could reduce brain damage following global forebrain ischemia. Transient global ischemia was induced in gerbils by occlusion of bilateral carotid arteries for 3 min. Propofol (50 mg/kg) was administered intraperitoneally 30 min before, immediately after, and at 1 h, 2 h, 6 h after occlusion. Thereafter, propofol was administered twice daily for three days. Treated animals were processed in parallel with ischemic animals receiving 10% intralipid as a vehicle or with sham-operated controls. In histologic findings, counts of viable neurons were made in the pyramidal cell layer of the hippocampal CA1 area 4 days after ischemia. The number of viable neurons in the pyramidal cell layer of CA1 area was similar in animals treated with a vehicle or a subanesthetic dose of propofol. In terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick end-labeling (TUNEL) assay, semiquantitative analysis of dark-brown neuronal cells was made in the hippocampal CA1 area. There was no significant difference in the degree of TUNEL staining in the hippocampal CA1 area between vehicle-treated and propofol-treated animals. These results show that subanesthetic dose of propofol does not reduce delayed neuronal cell death following transient global ischemia in Mongolian gerbils.
