Intracellular Ca2+ Mobilization and Beta-hexosaminidase Release Are Not Influenced by 60 Hz-electromagnetic Fields (EMF) in RBL 2H3 Cells.
10.4196/kjpp.2011.15.5.313
- Author:
Yeon Hee HWANG
1
;
Ho Sun SONG
;
Hee Rae KIM
;
Myoung Soo KO
;
Jae Min JEONG
;
Yong Ho KIM
;
Jeong Soo RYU
;
Uy Dong SOHN
;
Yoon Myoung GIMM
;
Sung Ho MYUNG
;
Sang Soo SIM
Author Information
1. College of Pharmacy, Chung-Ang University, Seoul 156-756, Korea. imss@cau.ac.kr
- Publication Type:Original Article
- Keywords:
EMF;
Ca2+ mobilization;
Exocytosis;
Beta-hexosaminidase
- MeSH:
beta-N-Acetylhexosaminidases;
Electromagnetic Fields;
Exocytosis;
Ionomycin;
Melitten;
Occupational Exposure;
Thapsigargin
- From:The Korean Journal of Physiology and Pharmacology
2011;15(5):313-317
- CountryRepublic of Korea
- Language:English
-
Abstract:
The effects of extremely low frequency electromagnetic fields (EMF) on intracellular Ca2+ mobilization and cellular function in RBL 2H3 cells were investigated. Exposure to EMF (60 Hz, 0.1 or 1 mT) for 4 or 16 h did not produce any cytotoxic effects in RBL 2H3 cells. Melittin, ionomycin and thapsigargin each dose-dependently increased the intracellular Ca2+ concentration. The increase of intracellular Ca2+ induced by these three agents was not affected by exposure to EMF (60 Hz, 1 mT) for 4 or 16 h in RBL 2H3 cells. To investigate the effect of EMF on exocytosis, we measured beta-hexosaminidase release in RBL 2H3 cells. Basal release of beta-hexosaminidase was 12.3+/-2.3% in RBL 2H3 cells. Exposure to EMF (60 Hz, 0.1 or 1 mT) for 4 or 16 h did not affect the basal or 1 microM melittin-induced beta-hexosaminidase release in RBL 2H3 cells. This study suggests that exposure to EMF (60 Hz, 0.1 or 1 mT), which is the limit of occupational exposure, has no influence on intracellular Ca2+ mobilization and cellular function in RBL 2H3 cells.
