The Protective Effect of Quercetin-3-O-beta-D-Glucuronopyranoside on Ethanol-induced Damage in Cultured Feline Esophageal Epithelial Cells.
10.4196/kjpp.2011.15.6.319
- Author:
Jung Hyun CHO
1
;
Sun Young PARK
;
Ho Sung LEE
;
Wan Kyunn WHANG
;
Uy Dong SOHN
Author Information
1. Department of Pharmacology, College of Pharmacy, Chung-Ang University, Seoul 156-756, Korea. udsohn@cau.ac.kr
- Publication Type:Original Article
- Keywords:
Flavonoid;
Hydrogen peroxide;
ERK;
Esophageal epithelial cell;
Ethanol
- MeSH:
Blotting, Western;
Cell Survival;
Epithelial Cells;
Ethanol;
European Union;
Fluoresceins;
Hydrogen Peroxide;
Interleukin-6;
NADPH Oxidase;
Onium Compounds;
Quercetin;
Rumex
- From:The Korean Journal of Physiology and Pharmacology
2011;15(6):319-326
- CountryRepublic of Korea
- Language:English
-
Abstract:
Quercetin-3-O-beta-D-glucuronopyranoside (QGC) is a flavonoid glucoside extracted from Rumex Aquaticus Herba. We aimed to explore its protective effect against ethanol-induced cell damage and the mechanism involved in the effect in feline esophageal epithelial cells (EEC). Cell viability was tested and 2',7'-dichlorofluorescin diacetate assay was used to detect intracellular H2O2 production. Western blotting analysis was performed to investigate MAPK activation and interleukin 6 (IL-6) expression. Exposure of cells to 10% ethanol time-dependently decreased cell viability. Notably, exposure to ethanol for 30 min decreased cell viability to 43.4%. When cells were incubated with 50 microM QGC for 12 h prior to and during ethanol treatment, cell viability was increased to 65%. QGC also inhibited the H2O2 production and activation of ERK 1/2 induced by ethanol. Pretreatment of cells with the NADPH oxidase inhibitor, diphenylene iodonium, also inhibited the ethanol-induced ERK 1/2 activation. Treatment of cells with ethanol for 30 or 60 min in the absence or presence of QGC exhibited no changes in the IL-6 expression or release compared to control. Taken together, the data indicate that the cytoprotective effect of QGC against ethanol-induced cell damage may involve inhibition of ROS generation and downstream activation of the ERK 1/2 in feline EEC.
