Cigarette Smoke Extract-induced Reduction in Migration and Contraction in Normal Human Bronchial Smooth Muscle Cells.
10.4196/kjpp.2011.15.6.397
- Author:
Chul Ho YOON
1
;
Hye Jin PARK
;
Young Woo CHO
;
Eun Jin KIM
;
Jong Deog LEE
;
Kee Ryeon KANG
;
Jaehee HAN
;
Dawon KANG
Author Information
1. Department of Physiology, Gyeongsang National University School of Medicine, Jinju 660-751, Korea. dawon@gnu.ac.kr, jheehan@gnu.ac.kr
- Publication Type:Original Article
- Keywords:
Bronchiole;
Cell migration;
Cigarette smoke extract;
Reactive oxygen species;
Smooth muscle
- MeSH:
Acetylcysteine;
Airway Remodeling;
Asthma;
Bronchioles;
Cell Death;
Cell Movement;
Cell Survival;
Contracts;
Emigration and Immigration;
Humans;
Lung Diseases;
Muscle, Smooth;
Myocytes, Smooth Muscle;
NF-kappa B;
Reactive Oxygen Species;
Smoke;
Tobacco Products;
Tumor Necrosis Factor-alpha
- From:The Korean Journal of Physiology and Pharmacology
2011;15(6):397-403
- CountryRepublic of Korea
- Language:English
-
Abstract:
The proliferation, migration, cytokine release, and contraction of airway smooth muscle cells are key events in the airway remodeling process that occur in lung disease such as asthma, chronic obstruction pulmonary disease, and cancer. These events can be modulated by a number of factors, including cigarette smoke extract (CSE). CSE-induced alterations in the viability, migration, and contractile abilities of normal human airway cells remain unclear. This study investigated the effect of CSE on cell viability, migration, tumor necrosis factor (TNF)-alpha secretion, and contraction in normal human bronchial smooth muscle cells (HBSMCs). Treatment of HBSMCs with 10% CSE induced cell death, and the death was accompanied by the generation of reactive oxygen species (ROS). CSE-induced cell death was reduced by N-acetyl-l-cysteine (NAC), an ROS scavenger. In addition, CSE reduced the migration ability of HBSMCs by 75%. The combination of NAC with CSE blocked the CSE-induced reduction of cell migration. However, CSE had no effect on TNF-alpha secretion and NF-kappaB activation. CSE induced an increase in intracellular Ca2+ concentration in 64% of HBSMCs. CSE reduced the contractile ability of HBSMCs, and the ability was enhanced by NAC treatment. These results demonstrate that CSE treatment induces cell death and reduces migration and contraction by increasing ROS generation in normal HBSMCs. These results suggest that CSE may induce airway change through cell death and reduction in migration and contraction of normal HBSMCs.
