The Effect of Methanol on the Structural Parameters of Neuronal Membrane Lipid Bilayers.
10.4196/kjpp.2012.16.4.255
- Author:
Hyung Jin JOO
1
;
Shin Ho AHN
;
Hang Rae LEE
;
Sung Woo JUNG
;
Chang Won CHOI
;
Min Seok KIM
;
Moon Kyoung BAE
;
In Kyo CHUNG
;
Soo Kyoung BAE
;
Hye Ock JANG
;
Il YUN
Author Information
1. Department of Dental Pharmacology and Biophysics, School of Dentistry and Research Institute for Oral Biotechnology, Yangsan Campus of Pusan National University, Yangsan 626-870, Korea.
- Publication Type:Original Article
- Keywords:
Annular lipid fluidity;
Membrane protein clustering;
Methanol;
Neuronal membranes;
Transbilayer lateral and rotational mobility
- MeSH:
Anisotropy;
Cell Membrane;
Cerebral Cortex;
Diphenylhexatriene;
Lipid Bilayers;
Membrane Lipids;
Membrane Proteins;
Membranes;
Methanol;
Neurons;
Palmitic Acids;
Phospholipids;
Proteins;
Stearic Acids
- From:The Korean Journal of Physiology and Pharmacology
2012;16(4):255-264
- CountryRepublic of Korea
- Language:English
-
Abstract:
The structures of the intact synaptosomal plasma membrane vesicles (SPMVs) isolated from bovine cerebral cortexs, and the outer and the inner monolayer separately, were evaluated with 1,6-diphenyl-1,3,5-hexatriene (DPH) and 1,3-di(1-pyrenyl)propane (Py-3-Py) as fluorescent reporters and trinitrophenyl groups as quenching agents. The methanol increased bulk rotational and lateral mobilities of SPMVs lipid bilayers. The methanol increased the rotational and lateral mobilities of the outer monolayers more than of the inner monolayers. n-(9-Anthroyloxy)stearic acid (n-AS) were used to evaluate the effect of the methanol on the rotational mobility at the 16, 12, 9, 6, and 2 position of aliphatic chains present in phospholipids of the SPMVs outer monolayers. The methanol decreased the anisotropy of the 16-(9-anthroyloxy)palmitic acid (16-AP), 12-(9-anthroyloxy)stearic acid (12-AS), 9-(9-anthroyloxy)stearic acid (9-AS), and 6-(9-anthroyloxy)stearic acid (6-AS) in the SPMVs outer monolayer but it increased the anisotropy of 2-(9-anthroyloxy)stearic acid (2-AS) in the monolayers. The magnitude of the increased rotational mobility by the methanol was in the order at the position of 16, 12, 9, and 6 of aliphatic chains in phospholipids of the outer monolayers. Furthermore, the methanol increased annular lipid fluidity and also caused membrane proteins to cluster. The important finding is that was far greater increase by methanol in annular lipid fluidity than increase in lateral and rotational mobilities by the methanol. Methanol alters the stereo or dynamics of the proteins in the lipid bilayers by combining with lipids, especially with the annular lipids. In conclusion, the present data suggest that methanol, in additions to its direct interaction with proteins, concurrently interacts with membrane lipids, fluidizing the membrane, and thus inducing conformational changes of proteins known to be intimately associated with membranes lipids.
