The Protective Effect of Eupatilin against Hydrogen Peroxide-Induced Injury Involving 5-Lipoxygenase in Feline Esophageal Epithelial Cells.
10.4196/kjpp.2012.16.5.313
- Author:
Jae Chun LIM
1
;
Sun Young PARK
;
Yoonjin NAM
;
Thanh Thao NGUYEN
;
Uy Dong SOHN
Author Information
1. Department of Pharmacology, College of Pharmacy, Chung-Ang University, Seoul 156-756, Korea. udsohn@cau.ac.kr
- Publication Type:Original Article
- Keywords:
5-lipoxygenase;
Esophageal epithelial cell;
Eupatilin;
Flavonoid;
Hydrogen peroxide
- MeSH:
Anthracenes;
Arachidonate 5-Lipoxygenase;
Artemisia;
Blotting, Western;
Cell Survival;
Epithelial Cells;
Flavonoids;
Hydrogen;
Hydrogen Peroxide;
Imidazoles;
Leukotriene B4;
Lipoxygenase;
MAP Kinase Signaling System;
Nordihydroguaiaretic Acid;
p38 Mitogen-Activated Protein Kinases;
Pyridines
- From:The Korean Journal of Physiology and Pharmacology
2012;16(5):313-320
- CountryRepublic of Korea
- Language:English
-
Abstract:
In this study, we focused to identify whether eupatilin (5,7-dihydroxy-3',4',6-trimethoxyflavone), an extract from Artemisia argyi folium, prevents H2O2-induced injury of cultured feline esophageal epithelial cells. Cell viability was measured by the conventional MTT reduction assay. Western blot analysis was performed to investigate the expression of 5-lipoxygenase by H2O2 treatment in the absence and presence of inhibitors. When cells were exposed to 600 microM H2O2 for 24 hours, cell viability was decreased to 40%. However, when cells were pretreated with 25~150 microM eupatilin for 12 hours, viability was significantly restored in a concentration-dependent manner. H2O2-treated cells were shown to express 5-lipoxygenase, whereas the cells pretreated with eupatilin exhibited reduction in the expression of 5-lipoxygenase. The H2O2-induced increase of 5-lipoxygenase expression was prevented by SB202190, SP600125, or NAC. We further demonstrated that the level of leukotriene B4 (LTB4) was also reduced by eupatilin, SB202190, SP600125, NAC, or nordihydroguaiaretic acid (a lipoxygenase inhibitor) pretreatment. H2O2 induced the activation of p38MAPK and JNK, this activation was inhibited by eupatilin. These results indicate that eupatilin may reduce H2O2-induced cytotoxicity, and 5-lipoxygenase expression and LTB4 production by controlling the p38 MAPK and JNK signaling pathways through antioxidative action in feline esophageal epithelial cells.
