Simultaneous Determination of Glutamate, Glycine, and Alanine in Human Plasma Using Precolumn Derivatization with 6-Aminoquinolyl-N-hydroxysuccinimidyl Carbamate and High-Performance Liquid Chromatography.
10.4196/kjpp.2012.16.5.355
- Author:
Qing Zhong LI
1
;
Qing Xian HUANG
;
Shu Cui LI
;
Mei Zi YANG
;
Bin RAO
Author Information
1. Institute of Pharmacy and Department of Pharmacology, School of Pharmacy, Binzhou Medical University, Yantai 264003, China. lqingzhong@yahoo.com.cn
- Publication Type:Original Article
- Keywords:
Amino acids;
AQC;
HPLC;
Plasma;
Ultraviolet detection
- MeSH:
Alanine;
Amino Acids;
Aminoquinolines;
Carbamates;
Chromatography, High Pressure Liquid;
Chromatography, Liquid;
Glutamic Acid;
Glycine;
Humans;
Perchloric Acid;
Plasma;
Proteins;
Quality Control
- From:The Korean Journal of Physiology and Pharmacology
2012;16(5):355-360
- CountryRepublic of Korea
- Language:English
-
Abstract:
A simple, sensitive and reproducible high-performance liquid chromatography (HPLC) method has been validated for determining concentrations of glutamate, glycine, and alanine in human plasma. Proteins in plasma were precipitated with perchloric acid, followed by derivatization with 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate (AQC). Simultaneous analysis of glutamate, glycine, and alanine is achieved using reversed-phase HPLC conditions and ultraviolet detection. Excellent linearity was observed for these three amino acids over their concentration ranges with correlation coefficients (r)>0.999. The intra- and inter-day precision were below 10%. This method utilizes quality control samples and demonstrates excellent plasma recovery and accuracy. The developed method has been successfully applied to measure plasma glutamate, glycine, and alanine in twenty volunteers.
