Modulation of Inwardly Rectifying K+ Channel by Intracellular and Extracellular pH in Bovine Aortic Endothelial Cells.
- Author:
Kyu Sang PARK
1
;
In Deok KONG
;
Joong Woo LEE
;
Hye whon RHIM
;
Young Chul KIM
;
In Suk SO
;
Ki Whan KIM
Author Information
1. Department of Physiology, Wonju College of Medicine, Yonsei University, Korea.
- Publication Type:Original Article
- Keywords:
Inwardly rectifying K+ channel;
Intracellular pH;
Extracellular pH
- MeSH:
Cell Membrane;
Endothelial Cells*;
Hydrogen-Ion Concentration*;
Membrane Potentials;
Membranes;
Patch-Clamp Techniques;
Protons
- From:The Korean Journal of Physiology and Pharmacology
2002;6(5):255-260
- CountryRepublic of Korea
- Language:English
-
Abstract:
The effects of intracellular and extracellular pH on the inwardly rectifying K+ (IRK) channel of the bovine aortic endothelial cells (BAECs) were examined using whole-cell patch-clamp technique. The IRK current, efficiently blocked by Ba2+ (200microM), is the most prominent membrane current in BAECs, which mainly determines the resting membrane potential. The expression of Kir2.1 was observed in BAECs using reverse transcriptase-polymerase chain reaction (RT-PCR) analysis. Intracellular alkalinization, elicited by the extracellular substitution of NaCl with NH4Cl (30 mM), significantly augmented the amplitude of IRK current. On the contrary, the amplitude of IRK current was attenuated by the Na-acetate (30 mM)-induced intracellular acidification. The changes in extracellular pH also closely modulated the amplitude of IRK current, which was decreased to 40.2+/-1.3% of control upon switching the extracellular pH to 4.0 from 7.4. The extracellular pH value for half-maximal inhibition (pK) of IRK current was 5.11. These results demonstrate that the activity of IRK channel in BAECs, probably Kir2.1, was suppressed by proton at both sides of plasma membrane.
