The p90rsk-mediated signaling of ethanol-induced cell proliferation in HepG2 cell line.
10.4196/kjpp.2016.20.6.595
- Author:
Han Sang KIM
1
;
Su Jin KIM
;
Jinhyung BAE
;
Yiyi WANG
;
Sun Young PARK
;
Young Sil MIN
;
Hyun Dong JE
;
Uy Dong SOHN
Author Information
1. Department of Pharmacology, College of Pharmacy, Chung-Ang University, Seoul 06974, Korea. udsohn@cau.ac.kr
- Publication Type:Original Article
- Keywords:
Bcl-2;
Ethanol;
Hepatocellular carcinoma;
NHE1;
p90rsk
- MeSH:
Blotting, Western;
Carcinoma, Hepatocellular;
Cell Proliferation*;
Cell Survival;
Enzyme-Linked Immunosorbent Assay;
Ethanol;
Hep G2 Cells*;
Humans;
Phosphorylation;
Phosphotransferases;
Protein Kinases;
Ribosomal Protein S6 Kinases
- From:The Korean Journal of Physiology and Pharmacology
2016;20(6):595-603
- CountryRepublic of Korea
- Language:English
-
Abstract:
Ribosomal S6 kinase is a family of serine/threonine protein kinases involved in the regulation of cell viability. There are two subfamilies of ribosomal s6 kinase, (p90rsk, p70rsk). Especially, p90rsk is known to be an important downstream kinase of p44/42 MAPK. We investigated the role of p90rsk on ethanol-induced cell proliferation of HepG2 cells. HepG2 cells were treated with 10~50 mM of ethanol with or without ERK and p90rsk inhibitors. Cell viability was measured by MTT assay. The expression of pERK1, NHE1 was measured by Western blots. The phosphorylation of p90rsk was measured by ELISA kits. The expression of Bcl-2 was measured by qRT-PCR. When the cells were treated with 10~30 mM of ethanol for 24 hour, it showed significant increase in cell viability versus control group. Besides, 10~30 mM of ethanol induced increased expression of pERK1, p-p90rsk, NHE1 and Bcl-2. Moreover treatment of p90rsk inhibitor attenuated the ethanol-induced increase in cell viability and NHE1 and Bcl-2 expression. In summary, these results suggest that p90rsk, a downstream kinase of ERK, plays a stimulatory role on ethanol-induced hepatocellular carcinoma progression by activating anti-apoptotic factor Bcl-2 and NHE1 known to regulate cell survival.
