Thyroid hormone-induced alterations of ryanodine and dihydropyridine receptor protein expression in rat hear.
- Author:
Hae Won KIM
1
;
Miyoung PARK
;
Eun Hee LEE
;
Hyoung Jin CHO
;
Hee Ran LEE
Author Information
1. Department of Pharmacology, University of Ulsan College of Medicine,388-1 Poongnapdong, Songpa-ku, Seoul, 138-736 South Korea.
- Publication Type:Original Article
- Keywords:
Heart;
Thyroid hormone;
Ryanodine receptor;
Dihydropyridine receptor
- MeSH:
Animals;
Calcium Channels, L-Type*;
Heart;
Hyperthyroidism;
Hypothyroidism;
Myocardium;
Rats*;
Relaxation;
RNA, Messenger;
Ryanodine Receptor Calcium Release Channel;
Ryanodine*;
Thyroid Gland*
- From:The Korean Journal of Physiology and Pharmacology
1999;3(3):329-337
- CountryRepublic of Korea
- Language:English
-
Abstract:
Thyroid hormone-induced cellular dysfunctions may be associated with changes in the intracellular Ca2+ concentration. The ryanodine receptor, a Ca2+ release channel of the SR, is responsible for the rapid release of Ca2+ that activates cardiac muscle contraction. In the excitation-contaction coupling cascade, activation of ryanodine receptors is initiated by the activity of sarcolemmal Ca2+ channels, the dihydropyridine receptors. In hyperthyroidism left ventricular contractility and relaxation velocity were increased, whereas these parameters were decreased in hypothyroidism. The mechanisms for these changes have been suggested to include alterations in the expression and/or activity levels of various proteins. In the present study, quantitative changes of ryanodine receptors and the dihydropyridine receptors, and the functional consequences of these changes in various thyroid states were investigated. In hyperthyroid hearts, (3H)ryanodine binding and ryanodine receptor mRNA levels were increased, but protein levels of ryanodine were not changed significantly. However, the above parameters were markedly decreased in hypothyroid hearts. In case of dihydropyridine receptor, there were a significant increase in the mRNA and protein levels, and (3H)nitrendipine binding, whereas no changes were observed in these parameters of hypothyroid hearts. Our findings indicate that hyperthyroidism is associated with increases in ryanodine receptor and dihydropyridine receptor expression levels, which is well correlated with the ryanodine and dihydropyridine binding. Whereas opposite changes occur in ryanodine receptor of the hypothyroid hearts.
