Effect of heme oxygenase induction by NO donor on the aortic contractility.
- Author:
Chang Kyun KIM
1
;
Uy Dong SOHN
;
Seok Yong LEE
Author Information
1. College of Pharmacy, Sungkyunkwan University, Suwon, South Korea. sylee@skku.ac.kr
- Publication Type:Original Article
- Keywords:
Heme oxygenase;
Carbon monoxide;
Vascular contractility;
NO
- MeSH:
Carbon Monoxide;
Catalysis;
Depression;
Endothelium;
Guanylate Cyclase;
Heme Oxygenase (Decyclizing)*;
Heme*;
Humans;
Methylene Blue;
Muscle, Smooth;
Myocytes, Smooth Muscle;
Oxidative Stress;
Phenylephrine;
Relaxation;
S-Nitroso-N-Acetylpenicillamine;
Tissue Donors*
- From:The Korean Journal of Physiology and Pharmacology
2001;5(1):87-92
- CountryRepublic of Korea
- Language:English
-
Abstract:
Carbon monoxide (CO) binds to soluble guanylate cyclase to lead its activation and elicits smooth muscle relaxation. The vascular tissues have a high capacity to produce CO, since heme oxygenase-2 (HO-2) is constitutively expressed in endothelial and smooth muscle cells, and HO-1 can be greatly up-regulated by oxidative stress. Moreover, the substrate of HO, heme, is readily available for catalysis in vascular tissue. Although the activation of heme oxygenase pathway under various stress conditions may provide a defence mechanism in compromised tissues, the specific role of HO-1-derived CO in the control of aortic contractility still remains to be elucidated. The present study was done to determine the effect of HO-1 induction on the aortic contractility. Thus, the effects of incubation of aortic tissue with S-nitroso-N-acetylpenicillamine (SNAP) for 1 hr on the aortic contractile response to phenylephrine were studied. The preincubation with SNAP resulted in depression of the vasoconstrictor response to phenylephrine. This effect was restored by HO inhibitor or methylene blue but not by NOS inhibitor. The attenuation of vascular reactivity by preincubation with SNAP was also revealed in endothelium-free rings. AlF4--evoked contraction in control did not differ from that in SNP-treated group. These results suggest that increased production of CO was responsible for the reduction of the contractile response to phenylephrine in aortic ring preincubated with SNAP and this effect of SNAP was independent on endothelium.
