Block of ATP-sensitive K+ channels expressed in Xenopus oocytes by dimethyl sulfoxide.
- Author:
Jin Bong PARK
1
;
Soo Wan CHAE
Author Information
1. Department of Pharmacology, Chonbuk National University Medical School, Jeonju, South Korea. soowan@moak.chonbuk.ac.kr
- Publication Type:Original Article
- Keywords:
Dimethyl sulfoxide;
ATP-sensitive K channel;
ROMK2;
Xenopus oocyte
- MeSH:
Adenosine Triphosphate;
Baths;
Dimethyl Sulfoxide*;
Oocytes*;
Sodium Azide;
Xenopus*
- From:The Korean Journal of Physiology and Pharmacology
2001;5(2):157-163
- CountryRepublic of Korea
- Language:English
-
Abstract:
The effects of dimethyl sulfoxide (DMSO) were studied in two groups of Xenopus oocytes, one expressing ATP sensitive K+ (KATP) channel comprised of sulfonylurea receptor SUR1 and inwardly rectifying K+ channel subunit Kir6.2, and the other expressing renal KATP channel ROMK2. At concentrations of 0.3~10% (vol/vol) DMSO inhibited whole cell Kir6.2/SUR1 currents elicited by bath application of sodium azide (3 mM) in a concentration-dependent manner. The inhibition constant and Hill coefficient were 2.93% and 1.62, respectively. ROMK2 currents, however, was not affected significantly by DMSO. The results support the idea that DMSO inhibits KATP channel expressed in Xenopus oocyte through a protein-specific mechanism(s) that remains to be further elucidated.
