Indomethacin induces apoptosis in NCI-H1299 human lung carcinoma cells.
- Author:
Bum Shik KIM
1
;
Soon Ae KIM
;
Mi Ja KIM
;
Hee Jae LEE
;
Seung Joon PARK
;
Jee Chang JUNG
;
Chang Ju KIM
;
Sung Vin YIM
;
Joo Ho CHUNG
Author Information
1. Department of Pharmacology, College of Medicine, Kyung Hee University, 1 Hoegi-dong, Dongdaemoon-gu, South Korea. jhchung@khu.ac.kr
- Publication Type:Original Article
- Keywords:
Chemoprevention;
Indomethacin;
Lung neoplasms;
Apoptosis
- MeSH:
Anti-Inflammatory Agents, Non-Steroidal;
Apoptosis*;
Cell Line;
Chemoprevention;
Colonic Neoplasms;
Humans*;
In Situ Nick-End Labeling;
Indomethacin*;
Lung Neoplasms;
Lung*
- From:The Korean Journal of Physiology and Pharmacology
2001;5(2):177-181
- CountryRepublic of Korea
- Language:English
-
Abstract:
Recently, nonsteroidal anti-inflammatory drugs (NSAIDs) have been found to be useful in the chemoprevention of colon cancer. To investigate whether indomethacin, an NSAIDs, induces apoptosis and thus assess the possibility of its application in the chemoprevention of human lung cancer, we have performed MTT assay, TUNEL assay, DAPI staining, and flow cytometric analysis using human lung carcinoma cell line NCI-H1299. Through morphological and biochemical analyses, it was demonstrated that NCI-H1299 cells treated with indomethacin (0.5 mM) exhibit classical apoptotic features. These results suggest that indomethacin induces apoptosis in NCI-H1299 cells and that NSAIDs, including indomethacin, may be a useful tool for the chemoprevention of human lung cancer.
