Antidiabetic activity and mechanisms of acarbose in KKAy mice.
- Author:
Young Lim KIM
1
;
Sung Hyun CHUNG
Author Information
1. School of Pharmacy, Kyung Hee University, Hoegi-dong 1, Dongdaemun-ku, Seoul, South Korea. suchung@khu.ac.kr
- Publication Type:Original Article
- Keywords:
Acarbose;
KKAy mouse;
Sucrase-isomaltase (SI);
sGLT1;
GLUT4
- MeSH:
Absorption;
Acarbose*;
Animals;
Blood Glucose;
Body Weight;
Gene Expression;
Glucose;
Glucose Transport Proteins, Facilitative;
Hyperglycemia;
Insulin;
Mice*;
Models, Animal;
Muscle, Skeletal;
Quadriceps Muscle;
RNA, Messenger;
Up-Regulation
- From:The Korean Journal of Physiology and Pharmacology
2001;5(2):183-188
- CountryRepublic of Korea
- Language:English
-
Abstract:
To elucidate antidiabetic effect and mechanism(s) of acarbose in a polygenic spontaneous hyperglycemic and hyperinsulinemic diabetic animal model, KKAy mice, acarbose was administered orally for 4 weeks and effects on body weight, plasma glucose and insulin levels, genetic expressions of intestinal sucrase-isomaltase (SI), sodium-glucose cotransporter (sGLT1) and glucose transporter in quadriceps muscle (GLUT4) were examined in this study. Although no differences in body weight were detected between control and acarbose-treated groups, plasma glucose level in acarbose-treated group was markedly reduced as compared to the control. In the mechanism study, acarbose downregulated the SI and SGLT1 gene expressions, and upregulated the GLUT4 mRNA and protein expressions when compared to the control group. In conclusion, the data obtained strongly implicate that acarbose can prevent the hyperglycemia in KKAy mice possibly through blocking intestinal glucose absorption by downregulations of SI and sGLT1 mRNA expressions, and upregulation of skeletal muscle GLUT4 mRNA and protein expressions.
