Effects of TGF-beta1 Ribbon Antisense on CCl4-induced Liver Fibrosis.
- Author:
Kyung Oh DOH
1
Author Information
- Publication Type:Original Article
- Keywords: Transforming growth factor-beta1; Liver cirrhosis; Ribbon antisense; Cationic peptide
- MeSH: Actins; Animals; Collagen; Fibrosis; Liver; Liver Cirrhosis; Mice; Muscles; RNA, Messenger; Transforming Growth Factor beta1; Veins
- From:The Korean Journal of Physiology and Pharmacology 2008;12(1):1-6
- CountryRepublic of Korea
- Language:English
- Abstract: Ribbon-type antisense oligonucleotide to TGF-beta1(TGF-beta1 RiAS) was designed and tested to prevent or resolve the fibrotic changes induced by CCl4 injection. When Hepa1c1c7 cells were transfected with TGF-beta1 RiAS, the level of TGF-beta1 mRNA was effectively reduced. TGF-beta1 RiAS, mismatched RiAS, and normal saline were each injected to mice via tail veins. When examined for the biochemical effects on the liver, TGF-beta1 mRNA levels were significantly reduced only in the TGF-beta1 RiAS-treated group. The results of immunohistochemical studies showed that TGF-beta1 RiAS prevented the accumulation of collagen and alpha-smooth muscle actin, but could not resolve established fibrosis. These results indicate that ribbon antisense to TGF-beta1 with efficient uptake can effectively prevent fibrosis of the liver.
