Effects of prostaglandin F2alpha on membrane potentials and K+ currents in rabbit middle cerebral arterial cells.
- Author:
Nari KIM
1
;
Jin HAN
;
Won Gue KIM
;
Euiyong KIM
Author Information
1. Department of Physiology and Biophysics, College of Medicine, Inje University, 633-165 Gaegeum-dong, Busanjin-gu, Busan, South Korea.
- Publication Type:Original Article
- MeSH:
Dinoprost*;
Kinetics;
Membrane Potentials*;
Membranes*;
Middle Cerebral Artery;
Myocytes, Smooth Muscle;
Patch-Clamp Techniques;
Vasoconstriction
- From:The Korean Journal of Physiology and Pharmacology
2000;4(4):301-309
- CountryRepublic of Korea
- Language:English
-
Abstract:
The purpose of our investigation was to examine the effects of prostaglandin F2alpha (PGF2alpha) on membrane potentials, Ca2+-activated K+ (KCa) channels, and delayed rectifier K+ (KV) channels using the patch-clamp technique in single rabbit middle cerebral arterial smooth muscle cells. PGF2alpha significantly hyperpolarized membrane potentials and increased outward whole-cell K+ currents. PGF2alpha increased open-state probability of KCa channels without the change of the open and closed kinetics. PGF2alpha increased the amplitudes of KV currents with a leftward shift of activation and inactivation curves and a decrease of activation time constant. Our results suggest that the activation of KCa and KV channels, at least in part, may lead to attenuate or counteract vasoconstriction by PGF2alpha in middle cerebral artery.
