Amperozide decreases cocaine-induced increase in behavior and immediate early gene expression in the dorsal striatum.
- Author:
Eun Sang CHOE
1
;
Jong Yeon KIM
Author Information
1. Department of Physiology, Yeungnam University College of Medicine, Taegu, South Korea. jykim@medical.yeungnam.ac.kr
- Publication Type:Original Article
- MeSH:
Cocaine;
Dopamine;
Gene Expression*;
In Situ Hybridization;
Receptors, Dopamine;
Ritanserin;
RNA, Messenger;
Serotonin
- From:The Korean Journal of Physiology and Pharmacology
2000;4(5):361-367
- CountryRepublic of Korea
- Language:English
-
Abstract:
Cocaine functions as indirect dopamine and serotonin (5-hydroxytryptamine, 5HT) agonists and induces genomic and behavioral alterations in the striatum. Previously we demonstrated that ritanserin, a 5HT2/1C receptor antagonist, is not responsible for cocaine-induced behavioral alterations and zif268 mRNA gene expression in the striatum (see the previous paper in this issue). In this study, it was hypothesized that dopamine and 5HT2/1C receptors are required for cocaine-induced behavioral alterations and c-fos and zif268 mRNA expression. This hypothesis was addressed by infusing amperozide which antagonizes both 5HT2/1C and dopamine receptors and was analyzed using the quantitative in situ hybridization histochemistry in vivo. Systemic injection of amperozide (5 mg/kg, s.c.) significantly blocked increase in behavior, c-fos and zif268 mRNA expression induced by 15 mg/kg cocaine, i.p., in the dorsal striatum. These data suggest that dopamine and 5HT2/1C receptors are necessary for cocaine-induced behavioral alterations and immediate early gene expression in the dorsal striatum.
