Different effects of dopamine on differential rotational mobility between inner and outer monolayer of synaptosomal plasma membrane vesicles isolated from bovine brain.
- Author:
Hyun Gang KIM
1
;
Chang Hwa CHOI
;
Inn Se KIM
;
In Kyo CHUNG
;
Goon Jae CHO
;
Hye Ock JANG
;
Il YUN
Author Information
1. Department of Dental Pharmacology and Biophysics, College of Dentistry and Research Institute for Oral Biotechnology, Pusan National University, Pusan, South Korea. iyun@hyowon.pusan.ac.kr
- Publication Type:Original Article
- Keywords:
Dopamine;
Neuronal membranes;
Transbilayer fluidity;
Fluorescent probe technique
- MeSH:
Anisotropy;
Brain*;
Cell Membrane*;
Diphenylhexatriene;
Dopamine*;
Fluorescence Polarization;
Membranes;
Neurons;
Plasma*
- From:The Korean Journal of Physiology and Pharmacology
2000;4(5):409-415
- CountryRepublic of Korea
- Language:English
-
Abstract:
Fluorescence polarization of 1,6-diphenyl-1,3,5-hexatriene (DPH) was used to evaluate the effects of dopamine cntdot HCl on the range of the rotatioanl mobility of bulk bilayer structure of the synaptosomal plasma membrane vesicles (SPMV) isolated from whole bovine brain. In a dose-dependent manner, dopamine decreased the anisotropy (gamma), limiting anisotropy (gammainfin) and order parameter (S) of DPH in the membranes. These indicate that dopamine increased the rotational mobility of the probe in the neuronal membranes. Cationic 1-(4-(trimethylammonio)-phenyl)-6-phenylhexa-1,3,5-hexatriene (TMA-DPH) and anionic 3-(p-(6-phenyl)-1,3,5-hexatrienyl)-phenylpropionic acid (PRO-DPH) were utilized to examine the range of transbilayer asymmetric rotational mobility of the neuronal membranes. Dopamine had a greater increasing effect on the mobility of the inner monolayer as compared to the outer monolayer of the neuronal membranes. It has been proven that dopamine exhibits a selective rather than nonselective fluidizing effect within the transbilayer domains of the SPMV.
