Differential Effects of Tautomycetin and Its Derivatives on Protein Phosphatase Inhibition, Immunosuppressive Function and Antitumor Activity.
10.4196/kjpp.2012.16.2.145
- Author:
Mingshan NIU
1
;
Yan SUN
;
Bo LIU
;
Li TANG
;
Rongguo QIU
Author Information
1. Research Center for Molecular Medicine, Dalian University of Technology, Dalian 116023, China. rqiu2001@yahoo.com, tangli63b@yahoo.com
- Publication Type:Original Article
- Keywords:
Tautomycetin;
Protein phosphatase;
Immunosuppressive activity;
Anticancer activity
- MeSH:
Cell Line;
Cell Proliferation;
Fermentation;
Furans;
Lipids;
Maleic Anhydrides;
MCF-7 Cells;
Structure-Activity Relationship
- From:The Korean Journal of Physiology and Pharmacology
2012;16(2):145-151
- CountryRepublic of Korea
- Language:English
-
Abstract:
In the present work, we studied the structure-activity relationship (SAR) of tautomycetin (TMC) and its derivatives. Further, we demonstrated the correlation between the immunosuppressive fuction, anticancer activity and protein phosphatase type 1 (PP1) inhibition of TMC and its derivatives. We have prepared some TMC derivatives via combinatorial biosynthesis, isolation from fermentation broth or chemical degradation of TMC. We found that the immunosuppressive activity was correlated with anticancer activity for TMC and its analog compounds, indicating that TMC may home at the same targets for its immunosuppressive and anticancer activities. Interestingly, TMC-F1, TMC-D1 and TMC-D2 all retained significant, albeit reduced PP1 inhibitory activity compared to TMC. However, only TMC-D2 showed immunosuppressive and anticancer activities in studies carried out in cell lines. Moreover, TMC-Chain did not show any significant inhibitory activity towards PP1 but showed strong growth inhibitory effect. This observation implicates that the maleic anhydride moiety of TMC is critical for its phosphatase inhibitory activity whereas the C1-C18 moiety of TMC is essential for the inhibition of tumor cell proliferation. Furthermore, we measured in vivo phosphatase activities of PP1 in MCF-7 cell extracts treated with TMC and its related compounds, and the results indicate that the cytotoxicity of TMC doesn't correlate with its in vivo PP1 inhibition activity. Taken together, our study suggests that the immunosuppressive and anticancer activities of TMC are not due to the inhibition of PP1. Our results provide a novel insight for the elucidation of the underlying molecular mechanisms of TMC's important biological functions.
