Hop Extract Produces Antinociception by Acting on Opioid System in Mice.
10.4196/kjpp.2012.16.3.187
- Author:
Soo Hyun PARK
1
;
Yun Beom SIM
;
Yu Jung KANG
;
Sung Su KIM
;
Chea Ha KIM
;
Su Jin KIM
;
Jee Young SEO
;
Su Min LIM
;
Hong Won SUH
Author Information
1. Department of Pharmacology, Institute of Natural Medicine, College of Medicine, Hallym University, Chuncheon 200-702, Korea. hwsuh@hallym.ac.kr
- Publication Type:Original Article
- Keywords:
Hop;
Antinociception;
Inflammatory pain;
Opioid receptor
- MeSH:
Animals;
Formaldehyde;
Glutamic Acid;
Humulus;
Injections, Spinal;
Methysergide;
Mice;
Mice, Inbred ICR;
Naloxone;
Reaction Time;
Receptors, Opioid;
Serotonin;
Substance P;
Yohimbine
- From:The Korean Journal of Physiology and Pharmacology
2012;16(3):187-192
- CountryRepublic of Korea
- Language:English
-
Abstract:
In the present study, the antinociceptive profiles of hop extract were characterized in ICR mice. Hop extract administered orally (from 25 to 100 mg/kg) showed an antinociceptive effect in a dose-dependent manner as measured in the acetic acid-induced writhing test. Antinociceptive action of hop extract was maintained at least for 60 min. Moreover, cumulative response time of nociceptive behaviors induced with intraplantar formalin injection was reduced by hop extract treatment during the 2nd phases. Furthermore, the cumulative nociceptive response time for intrathecal injection of substance P (0.7 microg) or glutamate (20 microg) was diminished by hop extract. Intraperitoneal pretreatment with naloxone (an opioid receptor antagonist) attenuated antinociceptive effect induced by hop extract in the writhing test. However, methysergide (a 5-HT serotonergic receptor antagonist) or yohimbine (an alpha2-adrenergic receptor antagonist) did not affect antinociception induced by hop extract in the writhing test. Our results suggest that hop extract shows an antinociceptive property in various pain models. Furthermore, the antinociceptive effect of hop extract may be mediated by opioidergic receptors, but not serotonergic and alpha2-adrenergic receptors.
