Development of Estimation Methods of Skin Oxidation and Evaluation of Anti-Oxidative Effects of Genistein in Topical Formulations.
10.4196/kjpp.2012.16.3.205
- Author:
Seong Yeon KIM
1
;
Yeon Joo NA
;
Dongju KIM
;
Yeongseok KIM
;
Hyeong Min KIM
;
Sung Ha HWANG
;
Jiyeon KWAK
;
Hyo Jeong KUH
;
Jaehwi LEE
Author Information
1. College of Pharmacy, Chung-Ang University, Seoul 156-756, Korea. jaehwi@cau.ac.kr
- Publication Type:Original Article
- Keywords:
Oxidation;
Skin;
Hydrogen peroxide;
Genistein
- MeSH:
Citrus sinensis;
Genistein;
Hydrogen Peroxide;
Hydrogen-Ion Concentration;
Phenols;
Skin;
Sulfoxides
- From:The Korean Journal of Physiology and Pharmacology
2012;16(3):205-209
- CountryRepublic of Korea
- Language:English
-
Abstract:
The objective of the present study was to establish the method of measurement of hydrogen peroxide and to estimate the anti-oxidative effect of genistein in the skin. UVB induced skin oxidation and anti-oxidative effect of genistein formulations were evaluated by determining levels of hydrogen peroxide. The mechanism involved in the determination of hydrogen peroxide is based on a color reaction between ferric ion (Fe3+) and xylenol orange, often called FOX assay and subsequent monitoring of absorbance values of the reactant at 540 nm. The reaction was to some extent pH-dependent and detection sensitivity was greatest at pH 1.75. Genistein liposomal gel demonstrated better anti-oxidative effect with regard to lowering hydrogen peroxide levels elevated by UVB irradiation compared to genistein-suspended gel. A linear relationship has been observed between anti-oxidative effect of genistein and drug deposition in the skin tissue. Genistein liposomal gel resulting in the localization of the drug in the deeper skin led to improved anti-oxidative effect compared to genistein gel. The suggested method for evaluation of oxidation of the skin can be used as a tool to screen effective anti-oxidative agents and their delivery systems acting on the skin.