alpha-Adrenergic and cholinergic receptor agonists modulate voltage-gated Ca2+ channels.
- Author:
Seung Yeol NAH
1
;
Jae Ha KIM
;
Cheon Ho KIM
Author Information
1. Department of Physiology, College of Veterinary Medicine.
- Publication Type:Original Article
- Keywords:
alpha-adrenergic receptor and muscarinic receptor;
Ca2+ currents;
Pertussis toxin-sensitive GTP binding proteins;
Rat trigeminal ganglion neurons
- MeSH:
Acetylcholine;
Adult;
Animals;
Atropine;
Carbachol;
Clonidine;
GTP-Binding Proteins;
Humans;
Neurons;
Norepinephrine;
Oxotremorine;
Pertussis Toxin;
Pharmacology;
Prazosin;
Rats;
Receptors, Muscarinic;
Trigeminal Ganglion;
Yohimbine
- From:The Korean Journal of Physiology and Pharmacology
1997;1(5):485-493
- CountryRepublic of Korea
- Language:English
-
Abstract:
We investigated the effect of alpha-adrenergic and cholinergic receptor agonists on Ca2+ current in adult rat trigeminal ganglion neurons using whole-cell patch clamp methods. The application of acetylcholine, carbachol, and oxotremorine (50 muM each) produced a rapid and reversible reduction of the Ca2+ current by 17+/-6%, 19+/-3% and 18+/-4%, respectively. Atropine, a muscarinic antagonist, blocked carbachol-induced Ca2+ current inhibition to 3 +/- 1%. Norepinephrine (50 muM) reduced Ca2+ current by 18 +/- 2%, while clonidine (50 muM), an alpha2-adrenergic receptor agonist, inhibited Ca2+ current by only 4 +/- 1%. Yohimbine, an alpha2-adrenergic receptor antagonist, did not block the inhibitory effect of norepinephrine on Ca2+ current, whereas prazosin, an alpha1-adrenergic receptor antagonist, attenuated the inhibitory effect of norepinephrine on Ca2+ current to 6 +/- 1%. This pharmacology contrasts with alpha2-adrenergic receptor modulation of Ca2+ channels in rat sympathetic neurons, which is sensitive to clonidine and blocked by yohimbine. Our data suggest that the modulation of voltage dependent Ca2+ channel by norepinephrine is mediated via an alpha1-adrenergic receptor. Pretreatment with pertussis toxin (250 ng/ml) for 16 h greatly reduced norepinephrine- and carbachol-induced Ca2+ current inhibition from 17 +/- 3% and 18 +/- 3% to 2 +/- 1% and 2 +/- 1%, respectively. These results demonstrate that norepinephrine, through an alpha1-adrenergic receptor, and carbachol, through a muscarinic receptor, inhibit Ca2+ currents in adult rat trigeminal ganglion neurons via pertussis toxin sensitive GTP-binding proteins.
