Increase of Intracellular Ca2+ Concentration Induced by Lysophosphatidylcholine in Murine Aortic Endothelial Cells.
- Author:
Mei Hong ZHU
1
;
Sung Jin PARK
;
Hyun Jin KIM
;
Dong Ki YANG
;
Suk Hyo SUH
;
Insuk SO
;
Ki Whan KIM
Author Information
1. Department of Physiology and Biophysics, Seoul National University College of Medicine, Seoul, Korea. kimkw@plaza.snu.ac.kr
- Publication Type:Original Article
- Keywords:
Oxidized low-density lipoprotein (ox-LDL);
Lysophosphatidylcholine (LPC);
2.5-di-tert- butylhydroquinone (BHQ);
Endothelial cell (EC)
- MeSH:
Adenosine Triphosphate;
Animals;
Cell Membrane;
Endothelial Cells*;
Lipoproteins;
Lysophosphatidylcholines*;
Mice;
RNA, Messenger
- From:The Korean Journal of Physiology and Pharmacology
2002;6(2):93-100
- CountryRepublic of Korea
- Language:English
-
Abstract:
Effects of oxidized low-density lipoprotein (ox-LDL), l-alpha-stearoyl-lysophosphatidylcholine (LPC), on intracellular Ca2+ concentration were examined in mouse endothelial cells by measuring intracellular Ca2+ concentration ([Ca2+]i) with fura 2-AM and reverse transcription-polymerase chain reaction (RT-PCR). LPC increased [Ca2+]i under the condition of 1.5 mM [Ca2+]o but did not show any effect under the nominally Ca2+-free condition. Even after the store depletion with 30microM 2,5-di-tert- butylhydroquinone (BHQ) or 30microM ATP, LPC could still increase the [Ca2+]i under the condition of 1.5 mM [Ca2+]o. The time required to increase [Ca2+]i (about 1 minute) was longer than that for ATP-induced [Ca2+]i increase (10-30 seconds). LPC-induced [Ca2+]i increase was completely blocked by 1microM La3+. Transient receptor potential channel(trpc) 4 mRNA was detected with RT-PCR. From these results, we suggest that LPC increased [Ca2+]i via the increase of Ca2+ influx through the Ca2+ routes which exist in the plasma membrane.
