Analysis of a Sphingosine 1-phosphate Receptor hS1P3 in Rat Hepatoma Cells.
- Author:
Dong Soon IM
1
Author Information
1. Laboratory of Pharmacology, College of Pharmacy, Pusan National University, Busan, Korea. imds@pusan. ac.kr
- Publication Type:Original Article
- Keywords:
Sphingosine 1-phosphate;
Lysophosphatidic acid;
G protein-coupled receptor;
S1P3;
cAMP;
MAP kinase;
Proliferation
- MeSH:
Adenylyl Cyclases;
Animals;
Carcinoma, Hepatocellular*;
Cell Line;
Cell Membrane;
Cell Proliferation;
DNA;
GTP-Binding Proteins;
Humans;
Oocytes;
Phosphotransferases;
Rats*;
Receptors, Lysosphingolipid*;
Sphingosine*
- From:The Korean Journal of Physiology and Pharmacology
2002;6(3):139-142
- CountryRepublic of Korea
- Language:English
-
Abstract:
To examine intracellular signaling of human S1P3 (hS1P3), a sphingosine 1-phosphate (S1P) receptor in plasma membrane, hS1P3 DNA was transfected into RH7777 rat hepatoma cell line, and the inhibition of forskolin-induced cAMP accumulation and activation of MAP kinases by S1P were tested. In hS1P3 transformants, S1P inhibited forskolin-induced activation of adenylyl cyclase activity by about 80% and activated MAP kinases in dose-dependent and pertussis-toxin (PTX) sensitive manners. In oocytes expressing hS1P3 receptor, S1P evoked Cl conductance. These data suggested that PTX-sensitive G proteins are involved in hS1P3-mediated signaling, especially the positive action of S1P in cell proliferation. The potential advantages of rat hepatoma cells for the research of sphingosine 1-phosphate receptor are discussed.
