Identification of AMPK activator from twelve pure compounds isolated from Aralia Taibaiensis: implication in antihyperglycemic and hypolipidemic activities.
10.4196/kjpp.2017.21.3.279
- Author:
Yuwen LI
1
;
Jongsun PARK
;
Yin WU
;
Jia CUI
;
Na JIA
;
Miaomiao XI
;
Aidong WEN
Author Information
1. Department of Pharmacy, Xijing Hospital, Fourth Military Medical University, Xi' an 710032, P. R. China. aidongwen71@hotmail.com miaomiaoxi02@hotmail.com
- Publication Type:Original Article
- Keywords:
AMPK;
Antihyperglycemic activity;
Hypolipidemic activity;
T2DM;
28-O-β-D-glucopyranosyl ester (AT12)
- MeSH:
Acetyl-CoA Carboxylase;
Adenosine Triphosphate;
AMP-Activated Protein Kinases*;
Animals;
Aralia*;
Blood Glucose;
China;
Diabetes Mellitus;
Diabetes Mellitus, Type 2;
Metabolic Diseases;
Models, Animal;
Oxygen Consumption;
Phosphorylation;
Rats;
Saponins;
Triglycerides
- From:The Korean Journal of Physiology and Pharmacology
2017;21(3):279-286
- CountryRepublic of Korea
- Language:English
-
Abstract:
The root bark extract of Aralia taibaiensis is used traditionally for the treatment of diabetes mellitus in China. The total saponin extracted from Aralia Taibaiensis (sAT) has effective combined antihyperglycemic and hypolipidemic activities in experimental type 2 diabetic rats. However, the active compounds have not yet been fully investigated. In the present study, we examined effects of twelve triterpenoid saponins on AMP-activated protein kinase (AMPK) activation, and found that compound 28-O-β-D-glucopyranosyl ester (AT12) significantly increased phosphorylation of AMPK and Acetyl-CoA carboxylase (ACC). AT12 effectively decreased blood glucose, triglyceride (TG), free fatty acid (FFA) and low density lipoprotein-cholesterol (LDL-C) levels in the rat model of type 2 diabetes mellitus (T2DM). The mechanism by which AT12 activated AMPK was subsequently investigated. Intracellular ATP level and oxygen consumption were significantly reduced by AT12 treatment. The findings suggested AT12 was a novel AMPK activator, and could be useful for the treatment of metabolic diseases.
