Cytochrome P450 2E1 activity in a Korean population.
- Author:
Dou Hyun MUHN
1
;
Jie Min CHAE
;
Jae Yong BAHN
;
Hae Jung SONG
;
Hyung Kee KIM
;
Jun Tack KWON
;
Dong Ryul SOHN
Author Information
1. Department of Clinical Pharmacology, Soonchunhyang University, College of Medicine, Chonan 330-090, Republic of Korea.
- Publication Type:Original Article
- Keywords:
Cytochrome P45O 2El;
Chlorzoxazone;
Koreans
- MeSH:
Asian Continental Ancestry Group;
Chlorzoxazone;
Chromatography, Liquid;
Cytochrome P-450 CYP2E1*;
Cytochrome P-450 Enzyme System*;
Cytochromes*;
Humans;
Limit of Detection;
Pharmacogenetics;
Solvents;
Xenobiotics
- From:The Korean Journal of Physiology and Pharmacology
1997;1(5):597-602
- CountryRepublic of Korea
- Language:English
-
Abstract:
Cytochrome P450 2E1 (CYP2E1) is involved in the toxicity and carcinogenicity of a number of solvents and xenobiotics. Like the various types of oxidation pharmacogenetics, the activity of the enzyme shows a discernible interindividual and interethnic variation. However, no pharmacogenetic information on CYP2E1 polymorphism has been available from a Korean population. The aim of this study was to explore the pharmacogenetics of CYP2E1 polymorphism in a native Koreans after an oral 400 mg dose of chlorzoxazone administered to 128 subjects. Urine samples were collected during the subsequent 8-hour period and urinary concentrations of chlorzoxazone and 6-hydroxychlorzoxazone were determined by a high performance liquid chromatography with an ultraviolet detector. The limit of detection in the samples was found to be 0.5 mug/ml. The mean value of the 6-hydroxychlorzoxazone excreted in 8 hr urine expressed as the percentage was 48.2 13.8%. The frequency distribution of percentage of the administered dose excreted as the 6-hydroxy metabolite was unimodally distributed in the subjects studied. However, the values showed wide (7-fold) interindividual difference, ranged from 11.6% to 79.8% of the dose of chlorzoxazone. Thus, it was considered that the pharmacogenetic characteristics of CYP2E1 in a Korean population did not-represent multimodal distribution in the 6-hydroxychlorzoxazone excreted in 8-hr urine expressed as the percentage. And the activity of the CYP2E1 in a Korean population seemed to be less compared with that of the Caucasian subjects.
