Effects of cyclic nucleotides and glipizide on the cardiovascular response of baclofen in the rats.
- Author:
In Chul SHIN
1
;
Hyun Chul KOH
;
Jin Hee HA
Author Information
1. Department of Pharmacology, College of Medicine, Hanyang University, Seoul 133-791, South Korea.
- Publication Type:Original Article
- Keywords:
Baclofen;
Spinal cord;
cAMP;
cGMP;
Glipizide;
Blood pressure;
Heart rate
- MeSH:
8-Bromo Cyclic Adenosine Monophosphate;
Adenylyl Cyclases;
Animals;
Baclofen*;
Blood Pressure;
Catheters;
gamma-Aminobutyric Acid;
Glipizide*;
Heart Rate;
Humans;
Injections, Spinal;
Male;
Membranes;
Nucleotides, Cyclic*;
Punctures;
Rats*;
Rats, Sprague-Dawley;
Spinal Cord
- From:The Korean Journal of Physiology and Pharmacology
1997;1(6):647-655
- CountryRepublic of Korea
- Language:English
-
Abstract:
The purpose of present study is to investigate the influence of a spinal gamma-aminobutyric acid B(GABA|B) receptor on a central regulation of blood pressure (BP) and heart rate (HR), and to define its mechanism in the spinal cord. In urethane-anesthetized, d-tubocurarine-paralyzed and artificially ventilated male Sprague-Dawley rats, intrathecal administration of drugs were carried out using injection cannula (33-gauge stainless steel) through the guide cannula (PE 10) which was inserted intrathecally at lower thoracic level through the puncture of a atlantooccipital membrane. Intrathecal injection of an GABA|B receptor agonist, baclofen (30, 60, 100 nmol) decreased both BP and HR dose-dependently. Pretreatment with 8-bromo-cAMP (50 nmol), a cAMP analog, or glipizide (50 nmol), a ATP-sensitive K+ channel blocker, attenuated the depressor and bradycardic effects of baclofen (100 nmol), but not with 8-bromo-cGMP (50 nmol), a cGMP analog. These results suggest that the GABA|B receptor in the spinal cord plays an inhibitory role in central cardiovascular regulation and that this depressor and bradycardic actions are mediated by the decrease of cAMP via the inhibition of adenylate cyclase and the opening of K+ channel.
