Inhibitory effects of 2,6-di-tert-butyl-4-hydroxymethylphenol on asthmatic responses to ovalbumin challenge in conscious guinea pigs.
10.4196/kjpp.2018.22.1.81
- Author:
Seul Yong JEONG
1
;
Ji Yun LEE
Author Information
1. College of Pharmacy, Chung-Ang University, Seoul 06974, Korea. jylee98@cau.ac.kr
- Publication Type:Original Article
- Keywords:
2,6-di-tert-butyl-4-hydroxymethylphenol;
Immediate asthmatic response;
Late-phase asthmatic response;
PLA₂;
sRaw
- MeSH:
Airway Resistance;
Albuterol;
Animals;
Asthma;
Bronchoalveolar Lavage Fluid;
Cromolyn Sodium;
Dexamethasone;
Eosinophils;
Guinea Pigs*;
Guinea*;
Histamine;
Leukocytes;
Lung;
Neutrophils;
Ovalbumin*;
Phenol;
Phospholipases;
Tidal Volume;
Vegetables
- From:The Korean Journal of Physiology and Pharmacology
2018;22(1):81-89
- CountryRepublic of Korea
- Language:English
-
Abstract:
This study evaluated the anti-asthmatic activities of 2,6-di-tert-butyl-4-hydroxymethylphenol (DBHP) that is a potent phenolic antioxidant in edible vegetable oil. The effects of DBHP on bronchial asthma were evaluated by determining the specific airway resistance (sRaw) and tidal volume (TV) during the immediate asthmatic response (IAR) and the late-phase asthmatic response (LAR) in guinea pigs with aerosolized ovalbumin-induced asthma. Recruitment of leukocytes and the levels of biochemical inflammatory mediators were determined in the bronchoalveolar lavage fluids (BALFs), and histopathological surveys performed in lung tissues. DBHP significantly inhibited the increased sRaw and improved the decreased TV on IAR and LAR, and also inhibited recruitment of eosinophils and neutrophils into the lung, and release of biochemical inflammatory mediators such as histamine and phospholipase A₂ from these infiltrated leukocytes, and improved pathological changes. However, anti-asthmatic activities of DBHP at oral doses of 12.5 to 50 mg/kg was less than those of dexamethasone (5 mg/kg, p.o.) and cromoglycate (10 mg/kg, p.o.), but more potent or similar to that of salbutamol (5 mg/kg, p.o.). These results in the present study suggest that anti-asthmatic effects of DBHP in the guinea pigs model of OVA-induced asthmatic responses principally are mediated by inhibiting the recruitments of the leukocytes and the release of biochemical inflammatory mediators from these infiltrated leukocytes.
