Detection of Mitochondrial ATP-Sensitive Potassium Channels in Rat Cardiomyocytes.
- Author:
Dang Van CUONG
1
;
Nari KIM
;
Euiyong KIM
;
Youngsuk LEE
;
Hyunju KIM
;
Sunghyun KANG
;
Dae Young HUR
;
Hyun JOO
;
Young Shik PARK
;
Yong Geun HONG
;
Sangkyung LEE
;
Joonyong CHUNG
;
Daehyun SEOG
;
Jin HAN
Author Information
1. Department of Physiology and Biophysics, College of Medicine, and 2020 Cardiovascular Institute, Inje University, Busan 614-735, Korea. phyhanj@ijnc.inje. ac.kr
- Publication Type:Original Article
- Keywords:
Mitochondrial ATP-sensitive K+ channel;
Ischemic preconditioning
- MeSH:
Animals;
Blotting, Western;
Fluorescence;
Fluorescent Antibody Technique;
Ischemic Preconditioning;
KATP Channels*;
Microscopy, Confocal;
Mitochondria;
Muscle Cells;
Myocytes, Cardiac*;
Potassium;
Rats*
- From:The Korean Journal of Physiology and Pharmacology
2004;8(4):201-206
- CountryRepublic of Korea
- Language:English
-
Abstract:
Mitochondrial ATP-sensitive potassium (mitoKATP) channels play a role in early and late ischemic preconditioning. Nevertheless, the subunit composition of mitoKATP channels remains unclear. In this study, we investigated the subunit composition of mitoKATP channels in mitochondria isolated from rat cardiac myocytes. Mitochondria were visualized using the red fluorescence probe, Mitrotracker Red, while mitoKATP channels were visualized using the green fluorescence probe, glibenclamide-BODIPY. The immunofluorescence confocal microscopy revealed the presence of Kir6.1, Kir6.2 and SUR2 present in the cardiac mitochondria. Western blot analysis was carried to further investigate the nature of mitoKATP channels. For SUR proteins, a 140-kDa immunoreactive band that corresponded to SUR2, but no SUR1 was detected. For Kir6.2, three bands (~4, ~6, and ~0 kDa) were detected, and a specific ~6-kDa immunoreactive band corresponding to Kir6.1 was also observed. These observations suggest that the subunits of mitoKATP channels in rat myocytes include Kir6.1, Kir6.2, and a SUR2-related sulfonylurea-binding protein.
