Identification of a Marker Protein for Cardiac Ischemia and Reperfusion Injury by Two-Dimensional Gel Electrophoresis and Matrix-Assisted Laser Desorption Ionization Mass Spectrometry.
- Author:
Youngsuk LEE
1
;
Nari KIM
;
Hyunju KIM
;
Hyun JOO
;
Youngnam KIM
;
Daehoon JEONG
;
Dang Van CUONG
;
Euiyong KIM
;
Dae Young HUR
;
Young Shik PARK
;
Yong Geun HONG
;
Sangkyung LEE
;
Joonyong CHUNG
;
Daehyun SEOG
;
Jin HAN
Author Information
1. 2020 Cardiovascular Institute, Department of Physiology and Biophysics, College of Medicine, Inje University, Busan 614-735, Korea. phyhanj@ijnc.inje.ac.kr
- Publication Type:Original Article
- Keywords:
Myoglobin;
Ischemiareperfusion injury;
Cardiac injury marker;
Two-dimensional gel electrophoresis;
Matrix-assisted laser desorption ionization mass spectrometry
- MeSH:
Blotting, Western;
Digestion;
Electrophoresis, Gel, Two-Dimensional*;
Gels;
Heart;
Ischemia*;
Ischemic Preconditioning;
Mass Spectrometry*;
Myoglobin;
Reperfusion Injury*;
Reperfusion*;
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization;
Trypsin
- From:The Korean Journal of Physiology and Pharmacology
2004;8(4):207-211
- CountryRepublic of Korea
- Language:English
-
Abstract:
The purpose of the present study was to evaluate the expression of cardiac marker protein in rabbit cardiac tissue that was exposed to ischemic preconditioning (IPC), or ischemiareperfusion injury (IR) using two-dimensional gel electrophoresis (2DE) and matrix-assisted laser desorption ionization mass spectrometry (MALDI-MS). We compared 2DE gels of control (uninjured) cardiac tissue with those of IPC and IR cardiac tissue. Expression of one protein was detected in IR heart tissue, however the protein was not detected in the samples of control and IPC tissue. To further characterize the detected protein molecule, the protein in the 2D gel was isolated and subjected to trypsin digestion, followed by MALDI-MS. The protein was identified as myoglobin, which was confirmed also by Western blot analysis. These results are consistent with previous studies of cardiac markers in ischemic hearts, indicating myoglobin as a suitable marker of myocardial injury. In addition, the present use of multiple techniques indicates that proteomic analysis is an appropriate means to identify cardiac markers in studies of IPC and IR.
