Effects of Fetal Mesencephalic Cell Grafts on the Intrastriatal 6-hydroxydoapmine Lesioned Rats.
- Author:
Wan Seok JOO
1
;
Eunjoo NAM
;
Heh In IM
;
Jin Ah JUNG
;
Eun Sun LEE
;
Yu Jin HWANG
;
Yong Sik KIM
Author Information
1. Department of Pharmacology, College of Medicine, Seoul National University, Seoul 110 799, Korea. kimysu@plaza.snu.ac.kr
- Publication Type:Original Article
- Keywords:
Parkinson's disease;
6-Hydroxydopamine;
Transplantation;
Tyrosine hydroxylase;
Glial cell line-derived neurotrophic factor
- MeSH:
Animals;
Dopamine;
Dopaminergic Neurons;
Fetus;
Glial Cell Line-Derived Neurotrophic Factor;
Nerve Growth Factors;
Oxidopamine;
Parkinson Disease;
Rats*;
Substantia Nigra;
Transplantation;
Transplants*;
Tyrosine 3-Monooxygenase
- From:The Korean Journal of Physiology and Pharmacology
2004;8(5):245-251
- CountryRepublic of Korea
- Language:English
-
Abstract:
The effects of fetal mesencephalic cell grafts on the restoration of nigrostriatal dopaminergic function were studied in the intrastriatal 6-hydroxydopamine-lesioned rats. Four weeks after lesioning, transplantation of ventral mesencephalic cells from embryonic day 14 fetuses showed the number of tyrosine hydroxylase (TH) positive cells and fiber outgrowth in the grafted striatum, and significantly ameliorated symptomatic motor behavior of the animals, as determined by apomorphine-induced rotation. Furthermore, in substantia nigra pars compacta (SNc), the numbers of TH cells and fibers were markedly restored. Dopamine content of ipsilateral SNc was close to that of contralateral SNc (91.9 9.8%) in the transplanted animals, while the ratio was approximately 32% in sham-grafted animals. These results indicate that grafted cells restored the activity for the dopaminergic neurons located in SNc, although they were transplanted into striatum. In addition, we showed that the implanted fetal cells expressed high level of glial cell line-derived neurotrophic factor (GDNF), suggesting that the transplanted fetal cells might serve as a dopamine producer and a reservoir of neurotrophic factors. These results may be helpful in consideration of the therapeutic transplantation at early stage of PD.
