Vibrio vulnificus Cytolysin Forms Anion-selective Pores on the CPAE Cells, a Pulmonary Endothelial Cell Line.
- Author:
Bok Hee CHOI
1
;
Byung Hyun PARK
;
Yong Geun KWAK
Author Information
1. Department of Pharmacology, Chonbuk National University Medical School, Chonju, Chonbuk 561-180, Korea. ygkwak@chonbuk.ac.kr
- Publication Type:Original Article
- Keywords:
V. vulnificus;
Cytolysin;
Pore;
Ion selectivity
- MeSH:
Anions;
Ascorbic Acid;
Baths;
Cell Line;
Endothelial Cells*;
Ions;
Nucleotides;
Patch-Clamp Techniques;
Perforin*;
Permeability;
Tea;
Vibrio vulnificus*;
Vibrio*;
Virulence
- From:The Korean Journal of Physiology and Pharmacology
2004;8(5):259-264
- CountryRepublic of Korea
- Language:English
-
Abstract:
Cytolysin produced by Vibrio vulnificus has been incriminated as one of the important virulence determinants in V. vulnificus infection. Ion selectivity of cytolysin-induced pores was examined in a CPAE cell, a cell line of pulmonary endothelial cell, using inside-out patch clamp techniques. In symmetrical NaCl concentration (140 mM), intracellular or extracellular application of cytolysin formed ion-permeable pores with a single channel conductance of 37.5 4.0 pS. The pore currents were consistently maintained after washout of cytolysin. Replacement of Na in bath solution with monovalent ions (K, Cs or TEA ) or with divalent ions (Mg2, Ca2 ) did not affect the pore currents. When the NaCl concentration in bath solution was lowered from 140 to 60 and 20 mM, the reversal potential shifted from 0 to 11.8 and 28.2 mV, respectively. The relative permeability of the cytolysin pores to anions measured at 40 mV was Cl = NO2 > or = Br = I > SCN > acetate > isethionate > ascorbic acid > EDTA2, in descending order. The cytolysin-induced pore current was blocked by Cl channel blockers or nucleotides. These results indicate that V. vulnificus cytolysin forms anion-selective pores in CPAE cells.
