5-Hydroxytryptamine Generates Tonic Inward Currents on Pacemaker Activity of Interstitial Cells of Cajal from Mouse Small Intestine.
10.4196/kjpp.2011.15.3.129
- Author:
Pawan Kumar SHAHI
1
;
Seok CHOI
;
Dong Chuan ZUO
;
Cheol Ho YEUM
;
Pyung Jin YOON
;
Jun LEE
;
Young Dae KIM
;
Chan Guk PARK
;
Man Yoo KIM
;
Hye Rang SHIN
;
Hyun Jung OH
;
Jae Yeoul JUN
Author Information
1. Department of Physiology, College of Medicine, Chosun University, Gwangju 501-759, Korea. jyjun@chosun.ac.kr
- Publication Type:Original Article
- Keywords:
Interstitial cells of Cajal (ICC);
Intestinal motility;
Pacemaker currents;
5-hydroxytryptamine (5-HT)
- MeSH:
Animals;
Flavonoids;
Gastrointestinal Motility;
Imidazoles;
Interstitial Cells of Cajal;
Intestine, Small;
Mice;
Mitogen-Activated Protein Kinases;
para-Aminobenzoates;
Patch-Clamp Techniques;
Phenols;
Pyridines;
Receptor, Serotonin, 5-HT2B;
Receptors, Serotonin;
Receptors, Serotonin, 5-HT4;
Serotonin;
Sulfonamides
- From:The Korean Journal of Physiology and Pharmacology
2011;15(3):129-135
- CountryRepublic of Korea
- Language:English
-
Abstract:
In this study we determined whether or not 5-hydroxytryptamine (5-HT) has an effect on the pacemaker activities of interstitial cells of Cajal (ICC) from the mouse small intestine. The actions of 5-HT on pacemaker activities were investigated using a whole-cell patch-clamp technique, intracellular Ca2+ ([Ca2+]i) analysis, and RT-PCR in ICC. Exogenously-treated 5-HT showed tonic inward currents on pacemaker currents in ICC under the voltage-clamp mode in a dose-dependent manner. Based on RT-PCR results, we found the existence of 5-HT2B, 3, 4, and 7 receptors in ICC. However, SDZ 205557 (a 5-HT4 receptor antagonist), SB 269970 (a 5-HT7 receptor antagonist), 3-tropanylindole - 3 - carboxylate methiodide (3-TCM; a 5-HT3 antagonist) blocked the 5-HT-induced action on pacemaker activity, but not SB 204741 (a 5-HT2B receptor antagonist). Based on [Ca2+]i analysis, we found that 5-HT increased the intensity of [Ca2+]i. The treatment of PD 98059 or JNK II inhibitor blocked the 5-HT-induced action on pacemaker activity of ICC, but not SB 203580. In summary, these results suggest that 5-HT can modulate pacemaker activity through 5-HT3, 4, and 7 receptors via [Ca2+]i mobilization and regulation of mitogen-activated protein kinases.
