Myoplasmic Ca2+, Crossbridge Phosphorylation and Latch in Rabbit Bladder Smooth Muscle.
10.4196/kjpp.2011.15.3.171
- Author:
Young Don KIM
1
;
Min Hyung CHO
;
Seong Chun KWON
Author Information
1. Department of Internal Medicine, Gangnung Asan Hospital, Gangnung 210-711, Korea.
- Publication Type:Original Article
- Keywords:
Urinary bladder;
Myoplasmic [Ca2+];
MRLC phosphorylation;
CCh;
Latch
- MeSH:
Carbachol;
Contracts;
Kinetics;
Muscle, Smooth;
Myosin Light Chains;
Phosphorylation;
Urinary Bladder
- From:The Korean Journal of Physiology and Pharmacology
2011;15(3):171-177
- CountryRepublic of Korea
- Language:English
-
Abstract:
Tonic smooth muscle exhibit the latch phenomenon: high force at low myosin regulatory light chains (MRLC) phosphorylation, shortening velocity (Vo), and energy consumption. However, the kinetics of MRLC phosphorylation and cellular activation in phasic smooth muscle are unknown. The present study was to determine whether Ca(2+)-stimulated MRLC phosphorylation could suffice to explain the agonist- or high K(+)-induced contraction in a fast, phasic smooth muscle. We measured myoplasmic [Ca2+], MRLC phosphorylation, half-time after step-shortening (a measure of Vo) and contractile stress in rabbit urinary bladder strips. High K(+)-induced contractions were phasic at both 22degrees C and 37degrees C: myoplasmic [Ca2+], MRLC phosphorylation, 1/half-time, and contractile stress increased transiently and then all decreased to intermediate values. Carbachol (CCh)-induced contractions exhibited latch at 37degrees C: stress was maintained at high levels despite decreasing myoplasmic [Ca2+], MRLC phosphorylation, and 1/half-time. At 22degrees C CCh induced sustained elevations in all parameters. 1/half-time depended on both myoplasmic [Ca2+] and MRLC phosphorylation. The steady-state dependence of stress on MRLC phosphorylation was very steep at 37degrees C in the CCh- or K(+)-depolarized tissue and reduced temperature flattend the dependence of stress on MRLC phosphorylation compared to 37degrees C. These data suggest that phasic smooth muscle also exhibits latch behavior and latch is less prominent at lower temperature.
