Characteristics of 5-Hydroxytryptamine Receptors Involved in Contraction of Feline Ileal Longitudinal Smooth Muscle.
10.4196/kjpp.2011.15.5.267
- Author:
YiYi WANG
1
;
Sun Young PARK
;
Kyung Hoon OH
;
Youngsil MIN
;
Yun Jeong LEE
;
Seok Yong LEE
;
Uy Dong SOHN
Author Information
1. Department of Pharmacology, College of Pharmacy, Chung-Ang University, Seoul 156-756, Korea. udsohn@cau.ac.kr
- Publication Type:Original Article
- Keywords:
Ileum;
Longitudinal smooth muscle;
5-HT;
Contraction;
Relaxation
- MeSH:
Atropine;
Benzamides;
Contracts;
Gastrointestinal Tract;
Guanethidine;
Ileum;
Indoles;
Ketanserin;
Methysergide;
Morpholines;
Muscle Contraction;
Muscle, Smooth;
Muscles;
Ondansetron;
Receptors, Serotonin;
Receptors, Serotonin, 5-HT1;
Receptors, Serotonin, 5-HT3;
Receptors, Serotonin, 5-HT4;
Relaxation;
Serotonin;
Serotonin Receptor Agonists;
Sulfonamides;
Tetrodotoxin
- From:The Korean Journal of Physiology and Pharmacology
2011;15(5):267-272
- CountryRepublic of Korea
- Language:English
-
Abstract:
A number of studies have demonstrated that 5-hydroxytryptamine (5-HT) can induce muscle contraction or relaxation response and enhance secretion in the gastrointestinal tract via a multiplicity of 5-HT receptor subtypes. In the present study, we investigated the pharmacological characterization of the 5-HT-induced contractile response in longitudinal smooth muscle isolated from the feline ileum. Addition of 5-HT into muscle chambers enhanced the basal tone and spontaneous activity in a concentration-dependent manner. The neurotoxin tetrodotoxin did not alter the 5-HT-induced contraction of the longitudinal muscles. Neither atropine nor guanethidine affected the contraction. The 5-HT agonists, 5-methylserotonin hydrochloride and mosapride, also evoked concentration-dependent contractions. The 5-HT-induced contraction was enhanced by the 5HT2 receptor antagonist ketanserin and the 5-HT3 receptor antagonist ondansetron but was inhibited by the 5-HT1 receptor antagonist methysergide and 5-HT4 receptor antagonist GR113808. These results indicate that 5-HT1 and 5-HT4 receptors may mediate the contraction of the 5-HT-induced response and 5-HT2 and 5-HT3 receptors may mediate 5-HT-induced relaxation in feline ileal longitudinal smooth muscles.
