Human umbilical cord blood mesenchymal stem cells engineered to overexpress growth factors accelerate outcomes in hair growth.
10.4196/kjpp.2018.22.5.555
- Author:
Dong Ho BAK
1
;
Mi Ji CHOI
;
Soon Re KIM
;
Byung Chul LEE
;
Jae Min KIM
;
Eun Su JEON
;
Wonil OH
;
Ee Seok LIM
;
Byung Cheol PARK
;
Moo Joong KIM
;
Jungtae NA
;
Beom Joon KIM
Author Information
1. Department of Dermatology, College of Medicine, Chung-Ang University, Seoul 06973, Korea. pugokjebi@gmail.com, beomjoon74@gmail.com
- Publication Type:Original Article
- Keywords:
Alopecia;
Dermal papilla cell;
Hair growth;
IGFBP-1;
Stem cell;
Stem-cell therapy
- MeSH:
Alkaline Phosphatase;
Alopecia;
Cell Survival;
Coculture Techniques;
Fetal Blood*;
Hair Follicle;
Hair*;
Humans*;
In Vitro Techniques;
Insulin-Like Growth Factor Binding Protein 1;
Insulin-Like Growth Factor I;
Intercellular Signaling Peptides and Proteins*;
Mesenchymal Stromal Cells;
Regeneration;
Stem Cells*;
Umbilical Cord*;
Vascular Endothelial Growth Factor A
- From:The Korean Journal of Physiology and Pharmacology
2018;22(5):555-566
- CountryRepublic of Korea
- Language:English
-
Abstract:
Human umbilical cord blood mesenchymal stem cells (hUCB-MSCs) are used in tissue repair and regeneration; however, the mechanisms involved are not well understood. We investigated the hair growth-promoting effects of hUCB-MSCs treatment to determine whether hUCB-MSCs enhance the promotion of hair growth. Furthermore, we attempted to identify the factors responsible for hair growth. The effects of hUCB-MSCs on hair growth were investigated in vivo, and hUCB-MSCs advanced anagen onset and hair follicle neogeneration. We found that hUCB-MSCs co-culture increased the viability and up-regulated hair induction-related proteins of human dermal papilla cells (hDPCs) in vitro. A growth factor antibody array revealed that secretory factors from hUCB-MSCs are related to hair growth. Insulin-like growth factor binding protein-1 (IGFBP-1) and vascular endothelial growth factor (VEGF) were increased in co-culture medium. Finally, we found that IGFBP-1, through the co-localization of an IGF-1 and IGFBP-1, had positive effects on cell viability; VEGF secretion; expression of alkaline phosphatase (ALP), CD133, and β-catenin; and formation of hDPCs 3D spheroids. Taken together, these data suggest that hUCB-MSCs promote hair growth via a paracrine mechanism.
